红花寄生叶3种溶剂提取物清除自由基活性

doi:A

天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (6): 1127-1132.doi: A

红花寄生叶3种溶剂提取物清除自由基活性

赖京菁，陈炳华*，连俊蕊，周冰洁，肖义军

福建师范大学生命科学学院，福州 350108

收稿日期:2010-01-22 出版日期:2011-12-22 发布日期:2012-07-04
基金资助:
福建省大学生创新性实验计划项目（Fjnu2007-011）；福建师范大学大学生课外科技项目（BKL2009-074）；福建师范大学国家级生物学实验教学示范中心本科生创新性研究计划项目（2009ls007）

Free Radical Scavenging Activities of Three Solvent Extracts from Scurrula parasitica Leaves

LAI Jing-jing, CHEN Bing-hua*, LIAN Jun-rui, ZHOU Bing-jie, XIAO Yi-jun

College of Life Sciences, Fujian Normal University, Fuzhou 350108, China

Received:2010-01-22 Online:2011-12-22 Published:2012-07-04

摘要/Abstract

摘要： 采用4种方法测定了红花寄生（寄主桑树）叶的水、80%甲醇和80%丙酮提取物对自由基的清除活性，以芦丁和BHT为对照品。结果表明，3种提取物均具有较强的自由基清除能力，且表现出不同程度的量效依赖关系；在3种溶剂提取物中，80%甲醇提取物清除•OH和O2^•-活性最强，半清除率浓度ρ(_SC50)分别为0.212 mg/mL、0.139 mg/mL，而80%丙酮提取物则清除DPPH•和ABTS•⁺活性最强，ρ(_SC50)分别为0.198 mg/mL、0.580 mg/mL。此外，3种溶剂提取物经酸水解后，HPLC均检出槲皮素和山奈酚等2种黄酮醇苷元，其含量范围分别为39.90~73.03 mg/g、4.82~9.19 mg/g间。可见，槲皮素及其苷类衍生物是红花寄生清除自由基活性的主要作用成分。

关键词: 红花寄生叶, 溶剂提取物, 自由基清除活性, 槲皮素衍生物

Abstract: The free radical-scavenging activities of water,80% methanol and 80% acetone extracts from Scurrula parasitica leaves (parasitic on Morus alba) were determined and compared with rutin and bulylated hydroxytoluene (BHT) using four kinds of methods. The results showed that all the leaf extracts had strong free radical-scavenging capacity with varying degrees of efficacy on a dose-dependent manner. Among the three solvent extracts, the 80% methanol extract had the highest scavenging activities on •OH and O2^•– with the value of SC₅₀ 0.212 mg/mL and 0.139 mg/mL, respectively. The 80% acetone extract exhibited the highest scavenging activities on DPPH• and ABTS•⁺ with the value of SC₅₀ 0.198 mg/mL and 0.580 mg/mL, respectively. In addition, when the solvent extracts were hydrolysed by acid, flavonol aglycones of quercetin and kaempferol were identified by HPLC in all solvent extracts with concentration ranges between 39.90~73.03 mg/g and 4.82~9.19 mg/g, respectively. Therefore, quercetin and its derivatives are the main active components accounting for the free radical-scavenging activities of Scurrula parasitica.

Key words: Scurrula parasitica leaves, solvent extracts, free radical scavenging activity, quercetin derivatives

中图分类号:

Q949.741.5 Q946.83 R284.2

赖京菁，陈炳华，连俊蕊，周冰洁，肖义军. 红花寄生叶3种溶剂提取物清除自由基活性[J]. 天然产物研究与开发, 2011, 23(6): 1127-1132.

LAI Jing-jing, CHEN Bing-hua, LIAN Jun-rui, ZHOU Bing-jie, XIAO Yi-jun. Free Radical Scavenging Activities of Three Solvent Extracts from Scurrula parasitica Leaves[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2011, 23(6): 1127-1132.

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红花寄生叶3种溶剂提取物清除自由基活性

Free Radical Scavenging Activities of Three Solvent Extracts from Scurrula parasitica Leaves

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