关键词: 高效液相色谱法, 分离, 肝源性磷脂酰胆碱

Abstract: In this study an effective method for separating phosphatidylcholine (PC) molecules from swine liver was developed by using reversed-phase high performance liquid chromatography.The effect of organic modifiers,the composition of mobile phase and the flow rate on PC molecules separation were also investigated.The results showed that the separation process benefited by adding hexane and ammonium acetate into the mobile phase with methanol as the dominant solvent. The method of methanol-acetonitrile-water gradient elution did not perform well in separating the PC molecules.The RP-HPLC method for separating PC molecules on Kromasil C₁₈ chromatographic column (4.6mm×200mm,5μm) was established with good sensitivity and reproducibility, by using methanol-hexane-0.05mol/L ammonium acetate-glycerol（84:6:8:0.6,V/V）as the mobile phase at the flow rate of 1.0 ml/min, testing wavelength of 206 nm and column temperature of 35 ℃. This research lays a foundation for analyzing the structures of the PC molecules in the future.

Key words: high performance liquid chromatography, separation and purification, phosphatidylcholine from swine liver