高速逆流色谱法从白芍中分离纯化五没食子酰基葡萄糖

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (3): 303-306.doi: A

高速逆流色谱法从白芍中分离纯化五没食子酰基葡萄糖

宋学英¹，殷志爽²，赵逸松¹，王桥^1*

¹首都医科大学化学生物学与药学院，北京 100069；²北京双鹤药业股份有限公司，北京 100012

收稿日期:2011-05-10 出版日期:2012-03-26 发布日期:2012-03-31
基金资助:
北京市中医药科技项目（JJ2008-005）

Isolation and Purification of Pentagalloylglucose from Radix Paeoniae Alba by High-speed Counter-current Chromatography

SONG Xue-ying¹, YIN Zhi-shuang¹, ZHAO Yi-song¹, WANG Qiao^1*

¹School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China;²Beijing Double-drane Pharmaceutical Co., Ltd., Beijing 100012, China

Received:2011-05-10 Online:2012-03-26 Published:2012-03-31

摘要/Abstract

摘要： 本文建立高速逆流色谱（HSCCC）方法，从白芍粗提物中分离纯化五没食子酰基葡萄糖。分别采用正己烷-乙酸乙酯-甲醇-水体积比 0.5:5:1:5 及0.5:5:0.5:5 混合溶剂作为两相溶剂体系，上相为固定相，下相为流动相，转速为800 r/min，流速为2.0 mL/min，用HPLC检测及ESI-MS进行验证。经过两次HSCCC分离纯化，得到五没食子酰基葡萄糖纯度为95.7%。

关键词: 高速逆流色谱, 高效液相色谱, 白芍, 五没食子酰基葡萄糖

Abstract: High-speed count-current chromatography (HSCCC) was developed for separation and purification of pentagalloylglucose (PGG) from crude extract of Radix Paeoniae Alba by the twice-step separation. The two of biphasic systems were composed of n-hexane-ethyl acetate-methanol-water at a volume ratio of 0.5:5:1:5 and 0.5:5:0.5:5 in HSCCC, respectively. The upper organic phase was as the stationary phase and the lower aqueous phase as mobile phase. The flow rate was at 2.0 mL/min and revolution speed was at 800 rpm. The effluent was monitored with a UV detector at 280 nm. The HSCCC fractions collected were analyzed by HPLC and the target compound of PGG was separated with a purity of 95.7%. In this study, the HSCCC technique was shown to be a powerful method to purify PGG from crude extract of Radix Paeoniae Alba and could represent a new technological tool to be applicable to the preparation of PGG from this source of products.

Key words: high-speed counter-current chromatography, HPLC, Radix Paeoniae Alba, pentagalloylglucose

中图分类号: R284.2

宋学英，殷志爽，赵逸松，王桥. 高速逆流色谱法从白芍中分离纯化五没食子酰基葡萄糖[J]. 天然产物研究与开发, 2012, 23(3): 303-306.

SONG Xue-ying, YIN Zhi-shuang, ZHAO Yi-song, WANG Qiao. Isolation and Purification of Pentagalloylglucose from Radix Paeoniae Alba by High-speed Counter-current Chromatography[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(3): 303-306.

参考文献

1 Wang WC(王维聪),Wang C(王潮),Song XY(宋学英),et al.Determination of 1,2,3,4,6-penta-O-galloyl-D-gloucose in forty four kind of Chinese traditional medicines by HPLC.Chin J Chin Meter Med(中国中药杂志),2008,33:656-659.
2 Piao MJ,Kang KA,Zhang R,et al.Antioxidant properties of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose from Elaeocarpus sylvestris var.ellipticus.Food Chem,2009,115:412–418.
3 Pei Y,Chen ZP,Ju HQ,et al.Autophagy is involved in anti-viral activity of pentagalloylglucose (PGG) against Herpes simplex virus type 1 infection in vitro.Biochem Bioph Res Co,2011,405:186-191.
4 Mizushina Y,Zhang JH,Puglese A,et al.Anti-cancer gallotannin penta-O-galloyl-beta- D-glucose is a nanomolar inhibitor of select mammalian DNA polymerases.Biochem Pharmacol,2010,80:1125-1132.
5 Huh JE,Lee EO,Kim MS,et al.Penta-O-galloyl-beta-D-glucose suppresses tumor growth via inhibition of angiogenesis and stimulation of apoptosis:roles of cyclooxygenase-2 and mitogen-activated protein kinas pathways.Carcinogenesis,2005,26:1436-1445.
6 Oh GS,Pae HO,Oh H,et al.In vitro anti-proliferative effect of 1,2,3,4,6-penta-O-galloyl-beta-D-glucose on human hepatocellular carcinoma cell line,SK-HEP-1 cells.Cancer Lett,2001,174:17–24.
7 Lee SJ,Lee HM,Ji ST,et al.1,2,3,4,6-penta-O-galloyl-D-glucose blocks endothelial cell growth and tube formation through inhibition of VEGF binding to VEGF receptor.Cancer Lett,2004,208:89-94.
8 Yin ZS(殷志爽),Zhao WH(赵文华),Song XY(宋学英)et al.Determination of 1,2,3,4,6-penta-O-galloyl-D-glucose in radix paeoniae alba and radix paeoniae rubra with HPLC.J Cap Med Uni (首都医科大学学报),2008,29:601-604.
9 Cao XL (曹学丽).Isolation Technology and Application of High-speed Counter Current Chromatography(高速逆流色谱分离技术及应用).Beijing:Chemical Industry Press.2004:44.

[1]	陈巍, 杨海峰, 陈毓, 王豪, 陈晓兰. 基于多指标成分定量联合多元统计分析评价不同产地鹅不食草药材质量[J]. 天然产物研究与开发, 2024, 36(9): 1573-1583.
[2]	卢立欣, 郑磊, 李志明, 宋超. 不同产地佩兰多成分定量分析及质量差异性评价研究[J]. 天然产物研究与开发, 2024, 36(8): 1409-1419.
[3]	杨新雪, 梁进京, 董蕊. 柴胡-白芍单味、药对配方颗粒与传统汤剂化学成分及抗抑郁对比研究[J]. 天然产物研究与开发, 2024, 36(7): 1182-1190.
[4]	刘雨然, 郭梦佳, 杨立新, 瞿燕, 潘晓丽, 杨滨, 李化. 不同道地产区黄芩的主要成分和抗氧化活性的比较研究[J]. 天然产物研究与开发, 2024, 36(6): 919-929.
[5]	杨新雪, 梁进京, 董蕊. 柴胡-白芍药对配方颗粒指纹图谱的建立及多指标性成分含量测定[J]. 天然产物研究与开发, 2024, 36(4): 572-580.
[6]	张一宁, 于志琪, 屈施旭, 刘同歌, 张玉红. 火麻叶中大麻二酚提取工艺的响应面法优化及不同部位含量分布[J]. 天然产物研究与开发, 2024, 36(3): 369-376.
[7]	涂如霞, 宋小仙, 陈慧, 何沛霖, 张莉, 郭延垒, 刘剑毅. 基于UPLC-Q-TOF-MS与分子对接的鸡矢藤有效成分及潜在靶点探讨[J]. 天然产物研究与开发, 2023, 35(增刊2): 55-62.
[8]	张杉杉, 张浩, 肖依文, 马倩, 黄国宇, 朱笃. HPLC同步测定南丰蜜橘成熟过程中黄酮类物质及其抗氧化活性研究[J]. 天然产物研究与开发, 2023, 35(增刊1): 16-22.
[9]	吴小可, 陈静华, 于瑞涛. HPLC法测定全缘叶绿绒蒿根、茎、叶、花中总黄酮醇苷的含量[J]. 天然产物研究与开发, 2023, 35(增刊1): 61-65.
[10]	陈海杰, 周永逸, 薛佳, 邹立思, 吴楠, 袁嘉欢, 刘训红, 程建明. 基于UFLC-QTRAP-MS/MS技术分析三叶崖爬藤不同部位的多元活性成分[J]. 天然产物研究与开发, 2023, 35(8): 1322-1337.
[11]	杨晓东, 罗宇琴, 吴文平, 潘礼业, 刘晓琳, 庞伟, 邢菊玲, 李国卫. 基于UPLC特征图谱和一测多评法的马鞭草质量评价及其标准汤剂量值传递规律研究[J]. 天然产物研究与开发, 2023, 35(8): 1393-1401.
[12]	蔡汶甫, 王月, 金文芳, 杨丽, 王洁雪, 张志锋. 基于UPLC-Q-Exactive-Orbitrap-MS的羌药中甸灯台报春化学成分分析[J]. 天然产物研究与开发, 2023, 35(6): 986-996.
[13]	吴娟娟, 张加余, 史石磊, 连运河, 程远欣, 武亚明, 蔡兴福, 吴迪. 不同产地红花椒椒目的全组分分析[J]. 天然产物研究与开发, 2023, 35(5): 837-851.
[14]	梁晶, 樊国全, 方涛, 李进, 张军高, 周小云, 龚静云, 雷斌. 21种药用植物提取物的抑菌活性筛选及其化学成分分析[J]. 天然产物研究与开发, 2023, 35(4): 562-572.
[15]	张椿翊, 邓乔晟, 郭子晗, 乔中华, 黄小兰, 王森, 周游, 周浓. UPLC法同时测定不同种类桑枝中5种黄酮类成分含量[J]. 天然产物研究与开发, 2023, 35(3): 372-378.

高速逆流色谱法从白芍中分离纯化五没食子酰基葡萄糖

Isolation and Purification of Pentagalloylglucose from Radix Paeoniae Alba by High-speed Counter-current Chromatography

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价