天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (4): 572-580.doi: 10.16333/j.1001-6880.2024.4.003

• 研究论文 • 上一篇    下一篇

柴胡-白芍药对配方颗粒指纹图谱的建立及多指标性成分含量测定

杨新雪，梁进京，董   蕊*   

  1. 吉林农业大学中药材学院，长春 130118
  • 出版日期:2024-04-28 发布日期:2024-04-28
  • 基金资助:
    国家中药标准化项目（ZYBZH-Y-ZL-25）

Establishment of fingerprint of Bupleurum Radix-Paeoniae Radix Alba formula granules and determination of multi-index components

YANG Xin-xue,LIANG Jin-jing,DONG Rui*   

  1. College of Chinese Materia Medica,Jilin Agricultural University,Changchun 130118,China
  • Online:2024-04-28 Published:2024-04-28

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摘要：

建立柴胡-白芍药对配方颗粒的HPLC指纹图谱、化学模式识别及多指标成分含量测定方法。采用COSMOSIL 5C18-MS-Ⅱ（250 mm×4.6 mm，5 μm）色谱柱；乙腈-0.1%磷酸水为流动相，梯度洗脱；体积流量1.0 mL/min；检测波长分别为210、230 nm；柱温30 ℃。建立HPLC指纹图谱，利用相似度评价、聚类分析（clustering analysis，CA）以及主成分分析（principal component analysis，PCA）对15批柴胡-白芍药对配方颗粒指纹图谱进行研究，同时测定4种指标性成分含量。本研究建立的指纹图谱共确定15个共有峰，并指认了其中的4种成分；15批柴胡-白芍药对配方颗粒指纹图谱与对照指纹图谱的相似度均>0.90；CA和PCA均可将15批柴胡-白芍药对配方颗粒大致分为2类；多指标成分含量测定方法符合方法学考察要求，4种成分的含量均有不同程度的差异。所建方法简单易行，可为柴胡-白芍药对配方颗粒的质量控制和临床用药提供参考。

关键词: 柴胡-白芍, 配方颗粒, 指纹图谱, 化学模式识别, 含量测定

Abstract:

To establish a method for HPLC fingerprint,chemical pattern recognition and multi-index component determination of Bupleurum Radix-Paeoniae Radix Alba (BR-PR) formula granules The chromatography was performed on COSMOSIL 5C18-MS-Ⅱ (250 mm×4.6 mm,5 μm) column.Acetonitrile-0.1% phosphoric acid water was mobile phase and gradient elution;Volume flow rate was 1.0 mL/min;The detection wavelengths were 210 nm and 230 nm;Column temperature was 30 ℃.The HPLC fingerprint was established,and the similarity evaluation,clustering analysis (CA) and principal component analysis (PCA) were used to study the fingerprint of 15 batches of BR-PR formula granules.The contents of four index components were determined simultaneously.The fingerprint established in this study identified 15 common peaks and identified four components of them.The similarity between the fingerprint of 15 batches of BR-PR formula granules medicine and the control fingerprint was all >0.90. Both CA and PCA could divide 15 batches of BR-PR formula granules into two categories.The multi-index component content determination method meets the requirements of methodological investigation,and the contents of four components are different in different degrees.The established method is simple and feasible,and can provide reference for the quality control and clinical use of BR-PR formula granules.

Key words: Bupleurum Radix-Paeoniae Radix Alba, formula granule, fingerprints, chemical pattern recognition, content determination

中图分类号:  R283.6

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