天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (2): 233-242.doi: 10.16333/j.1001-6880.2024.2.006

• 研究简报 • 上一篇    下一篇

基于HPLC指纹图谱结合一测多评法的葛花质量控制研究

张立军1，张新玥4，李   鹏3， 张转平1*，朱远锋1，刘   丽1，王   磊2，吴春燕1   

  1. 1安康市食品药品检验检测中心；2安康市天源植物提取有限公司，安康 725000；3商洛市药品检验所，商洛 726000；4北京中医药大学药学院，北京 100029
  • 出版日期:2024-02-23 发布日期:2024-02-23
  • 基金资助:
    陕西省社会发展科技攻关项目（2015SF286）；安康市社会发展科技攻关项目（AK2021-SF-01）；陕西省药品科学监管和监管科学研究项目（SXYJ202317）

Quality control of Puerariae Flos based on HPLC fingerprint and quantitative analysis of multi-components with single-marker method

ZHANG Li-jun1,ZHANG Xin-yue4,LI Peng3,ZHANG Zhuan-ping1*,ZHU Yuan-feng1,LIU Li1,WANG Lei2,WU Chun-yan1   

  1. 1Ankang Inspection and Detection Center of Food and Drug Control; 2Ankang Tianyuan Plant Extraction Co.,Ltd., Ankang 725000,China;3Shangluo Drug Inspection Center,Shangluo 726000,China;4Pharmacy College,Beijing University of Chinese Medicine,Beijing 100029,China
  • Online:2024-02-23 Published:2024-02-23

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摘要： 建立葛花药材的指纹图谱，并同时建立其中10个主要黄酮类成分含量的一测多评分析方法（QAMS），用于不同产地葛花药材整体质量评价。采用高效液相色谱法（HPLC）对不同产地葛花中主要化学成分进行分析，确定最佳色谱条件，建立指纹图谱；在此基础上，以葛花苷为参照峰，建立QAMS测定葛花10个黄酮类成分含量；并与外标法（ESM）测定结果比较，验证QAMS的准确性。建立了葛花药材指纹图谱，20批葛花和10批粉葛花药材的相似度均大于0.85，标定共有峰分别为18和27个。以葛花苷为参照峰，各个成分校正因子的重复性、耐用性均良好；应用一测多评法测定10个成分的含量，与外标法测定结果比较，含量无明显差异（P＞0.05），相对偏差＜3.0%。不同产地来源的葛花药材中10个成分含量均存在较大差异，说明应提高葛花药材的质量控制标准。所建立的指纹图谱结合一测多评分析方法简便、可行，可客观、有效的用于不同产地葛花药材质量评价，可以为葛花质量标准修订、优质葛花种质资源的选育和育种提供参考。

关键词: 葛花, 粉葛花, 指纹图谱, 一测多评, 不同产区, 质量评价

Abstract:

To establish the HPLC fingerprint of Puerariae Flos and quantitative analysis of multi-components by single-marker (QAMS) for ten main flavonoids,in order to quality evaluation of Puerariae Flos in different regions.HPLC was used to analyze the chemical components from Puerariae Flos.The chromatographic conditions were optimized,and the fingerprint was established.Kakkalide was used as the reference compound to establish QAMS method for the determination of the main effective components in Puerariae Flos,and compared with the results of external standard method (ESM) to verify the accuracy of this method.The common mode for Pueraria lobata (Willd.) Ohwi (GH) and Pueraria thomsonii Benth.(FGH) fingerprint was established;the similarity were greater than 0.85 in 20 batches of GH and 10 batches of FGH;18 and 27 common fingerprint peaks were identified.Kakkalide was used as the reference compound.The repeatability and durability of relative correction factor for each component was good.There was no significant difference (P＞0.05) between the measured values of QAMS method and ESM,relative deviation <3%.The components contents in Puerariae Flos from different origins were both greatly different.The results demonstrated quality control standard of Puerariae Flos should be improved.The establishment of fingerprint and QAMS method in this study was rapid,simple,objectively and effectively and full quality evaluation of Puerariae Flos,which can provide the references for the breeding and breeding high-quality germplasm resources and improving the quality control standard of Puerariae Flos when revising the Puerariae Flos.

Key words: Pueraria lobata (Willd.) Ohwi, Pueraria thomsonii Benth., fingerprint, QAMS, different regions, quality evaluation

中图分类号:  O657.72 R931.5

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