骨碎补UPLC指纹图谱与人骨肉瘤细胞MG63中碱性磷酸酶活性的谱效关系研究

doi:10.16333/j.1001-6880.2024.2.012

天然产物研究与开发 ›› 2024, Vol. 36 ›› Issue (2): 293-302.doi: 10.16333/j.1001-6880.2024.2.012

骨碎补UPLC指纹图谱与人骨肉瘤细胞MG63中碱性磷酸酶活性的谱效关系研究

陈香丽，张慧敏，张紫佳*

上海中医药大学中药研究所中药标准化教育部重点实验室，上海 201203

出版日期:2024-02-23 发布日期:2024-02-23
基金资助:
国家重点研发计划子任务（2019YFC1711004）

Study on the spectrum-effect relationship between UPLC fingerprint and alkaline phosphatase activity in osteosarcoma MG63 cell of Drynariae Rhizoma

CHEN Xiang-li,ZHANG Hui-min,ZHANG Zi-jia*

The MOE Key Laboratory for Standardization of Chinese Medicines,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

Online:2024-02-23 Published:2024-02-23

摘要/Abstract

摘要： 建立骨碎补生品和砂烫品提取物的UPLC指纹图谱，并研究骨碎补提取物与人骨肉瘤细胞MG63中碱性磷酸酶（ALP）活性之间的谱效关系。采用UPLC法建立骨碎补生品和烫品的指纹图谱并通过UPLC-Q-TOF MS法对其化学成分进行鉴别；通过测定MG63细胞中ALP活性检测骨碎补提取物对MG63细胞成骨分化的影响；采用灰色关联度分析、双变量相关性分析以及偏最小二乘回归分析法分析骨碎补生品和烫品的UPLC特征指纹峰与其促ALP活性之间的谱效关系。在标定的12个共有峰中，确定了4、5、12号峰与ALP活性呈正相关，其对应的物质依次是咖啡酸-4-O-β-D-吡喃葡萄糖苷、儿茶素7-葡糖苷、柚皮苷，为深入研究骨碎补的促成骨分化活性物质基础提供依据。

关键词: 骨碎补, UPLC指纹图谱, 碱性磷酸酶活性, 谱效关系, 人骨肉瘤细胞MG63

Abstract:

To establish a UPLC fingerprint of the extract of Drynariae Rhizoma before and after processing,and to study the spectrum-effect relationship between the extract of Drynariae Rhizoma and alkaline phosphatase (ALP) activity in osteosarcoma MG63 cell line.Using UPLC method to establish the fingerprint of raw materials and processing slices of Drynariae Rhizoma and identified their chemical components by UPLC-Q-TOF MS;The ALP activity was measured to determine the effect of Drynariae Rhizoma extract on osteosarcoma MG63 cell line;Grey correlation analysis,bivariate correlation analysis,and partial least square regression analysis were used to analyze the spectrum-effect relationship between the UPLC characteristic fingerprint peaks and ALP activity of the raw and processed products of Drynariae Rhizoma.Among the 12 characteristic fingerprint peaks calibrated,it was determined that peaks 4,5 and 12 were positively correlated with ALP activity,and the corresponding substances were caffeic acid 4-O-β-D-glucopyranoside,catechin 7-glucoside and naringin.It provides a reference for in-depth research on the active material basis of Drynariae Rhizoma in promoting osteogenic differentiation effect.

Key words: Drynariae Rhizoma, UPLC fingerprint, alkaline phosphatase activity, spectrum-effect relationship, osteosarcoma MG63 cell line

中图分类号: R284.1

陈香丽, 张慧敏, 张紫佳. 骨碎补UPLC指纹图谱与人骨肉瘤细胞MG63中碱性磷酸酶活性的谱效关系研究[J]. 天然产物研究与开发, 2024, 36(2): 293-302.

CHEN Xiang-li, ZHANG Hui-min, ZHANG Zi-jia.

Study on the spectrum-effect relationship between UPLC fingerprint and alkaline phosphatase activity in osteosarcoma MG63 cell of Drynariae Rhizoma

[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2024, 36(2): 293-302.

[1]	杜兰哲, 范倩, 蔡盛康, 殷洪林, 吴晓纯, 陶晨璐, 孙冬梅, 丁青, 朱德全. 基于UPLC指纹图谱和含量测定的六月雪质量评价研究[J]. 天然产物研究与开发, 2023, 35(增刊2): 63-71.
[2]	李振雨, 刘晓霞, 冯涌微, 位翠杰, 何民友, 邓淙友, 陈向东, 孙冬梅. 姜黄标准汤剂UPLC指纹图谱的建立和4种成分定量分析研究[J]. 天然产物研究与开发, 2023, 35(5): 807-818.
[3]	李振雨, 梁月仪, 吕渭升, 黄爽, 杨洁, 卢晓莹, 陈向东, 孙冬梅. 马鞭草药材UPLC指纹图谱建立及指标性成分的测定[J]. 天然产物研究与开发, 2023, 35(4): 551-561.
[4]	张传洋, 杨璐嘉, 陈翠平, 王辉, 赵元静, 邓放. 锦灯笼UPLC-Q-Orbitrap HRMS指纹图谱及抗菌活性的谱效关系[J]. 天然产物研究与开发, 2023, 35(10): 1699-1707.
[5]	杨文惠, 吕渭升, 孙冬梅, 罗文汇, 杨洁, 何民友, 潘礼业, 李振雨. UPLC指纹图谱结合多元统计分析的吴茱萸与制吴茱萸成分变化研究[J]. 天然产物研究与开发, 2022, 34(8): 1301-1310.
[6]	李振雨, 周湘媛, 马懿飞, 何民友, 刘晓霞, 陈向东, 孙冬梅, 罗文汇. 基于UPLC指纹图谱和多指标成分定量的不同产地白茅根标准汤剂质量评价研究[J]. 天然产物研究与开发, 2022, 34(11): 1900-1910.
[7]	武鹤婷, 赛那瓦尔·芒思尔, 高彦华, 信学雷, 宁慧霞. 红花降糖活性成分谱效关系研究[J]. 天然产物研究与开发, 2021, 33(2): 188-197.
[8]	王子怡, 穆文成, 李艳荣, 洪霞, 潘海峰. 山楂叶标准汤剂指纹图谱及抗氧化活性谱效关系研究[J]. 天然产物研究与开发, 2020, 32(4): 557-566.
[9]	耿晓桐1，王丰青3，谢彩侠1,2，张苗1，陈志红1，龚海燕1，雷敬卫1，张重义2. 基于HPLC指纹图谱技术的地黄块根抗氧化活性谱效关系研究[J]. 天然产物研究与开发, 2019, 31(11): 1864-1872.
[10]	赵赶1，何冠兰2，卢春远2，劳玲玲2，潘宇政3，周马林2，韦锦斌2*. 骨碎补总黄酮对免疫低下模型小鼠免疫功能的影响[J]. 天然产物研究与开发, 2016, 28(3): 438-445.

骨碎补UPLC指纹图谱与人骨肉瘤细胞MG63中碱性磷酸酶活性的谱效关系研究

Study on the spectrum-effect relationship between UPLC fingerprint and alkaline phosphatase activity in osteosarcoma MG63 cell of Drynariae Rhizoma

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价