海芦笋及其生物盐中多酚酸和绿原酸的定量分析

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (4): 494-497.doi: A

海芦笋及其生物盐中多酚酸和绿原酸的定量分析

李建伟^１，杨志²，刘晓满², 吴世华^2*

¹宁波天韵农业开发有限公司，宁波 315192； ²浙江大学生命科学学院思源天然药物与生物毒素研究中心，杭州310058

收稿日期:2010-11-30 出版日期:2012-04-26 发布日期:2012-05-16
基金资助:
宁波市科技计划项目（2007C10029）

Quantitative Analysis of Phenolic Acids and Chlorogenic Acid of Salicornia bigelovii Torr.

LI Jian-wei ¹, YANG Zhi², LIU Xiao-man ², WU Shi-hua^2*

¹Tianyun Agriculture Research and Development Ltd. Corp, Ningbo 315192, China ; ² Research Center of Siyuan Natural Pharmacy and Biotoxicology, Collage of Life Sciences, Zhejiang University, Hangzhou 310058, China

Received:2010-11-30 Online:2012-04-26 Published:2012-05-16

摘要/Abstract

摘要： 以绿原酸为对照品，利用紫外分光光度法和高效液相色谱法分别建立了测定海芦笋中多酚酸和绿原酸含量的方法。紫外分光光度法检测波长为338 nm；高效液相色谱法采用Zorbax Eclipse XDB-C18（4.6 mm × 150 mm，5um），以甲醇和0.5%冰醋酸水溶液梯度洗脱。结果发现海芦笋及其生物盐中含有大量的多酚酸和绿原酸，其中多酚酸含量分别为6.49 g/kg, 3.37 g/kg, 绿原酸含量分别为0.234 g/kg, 0.180 g/kg。同时也表明高效液相色谱法可用于海芦笋中多酚酸含量的确定。这两种简单快捷的定量分析多酚酸和绿原酸的方法，不仅可用于海芦笋及其生物盐产品的质量控制，而且也为海芦笋的进一步研究和开发提供了一定的依据。

关键词: 海芦笋, 总酚酸, 绿原酸, 紫外分光光度法, HPLC-DAD, 定量分析

Abstract: Two methods including the ultraviolet spectrophotometry and the HPLC-DAD had been developed to analyze quantitatively the total phenolic acids and chlorogenic acid of Salicornia bigelovii Torr, and its biosalt production. The ultraviolet spectrophotometry was set at 338 nm and chlorogenic acid was used as the reference substance. HPLC was performed on a Zorbax Eclipse XDB-C18 (4.6 mm × 150 mm, 5um) using a mobile phase of methanol and 0.5% acetic acid aqueous solution with a linear gradient. It was found that S. bigelovii contained high contents of phenolic acids and chlorogenic acid, and the contents of the total phenolic acids was 6.49 g/kg and 3.37 g/kg for S. bigelovii and its biosalt production, while the contents of chlorogenic acid was 0.234 g/kg and 0.180 g/kg. It was very efficient for quantitative analysis of the total phenolic acids and chlorogenic acid and could be used for quality control of S. bigelovii and its biosalt production.

Key words: Salicornia bigelovii Torr , polyphenols, chlorogenic acid, HPLC, quantitative analysis

中图分类号:

R917

李建伟，杨志，刘晓满, 吴世华. 海芦笋及其生物盐中多酚酸和绿原酸的定量分析[J]. 天然产物研究与开发, 2012, 23(4): 494-497.

LI Jian-wei, YANG Zhi, LIU Xiao-man, WU Shi-hua. Quantitative Analysis of Phenolic Acids and Chlorogenic Acid of Salicornia bigelovii Torr.[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(4): 494-497.

参考文献 1

Wu GQ(吴国泉),Huang MF(黄凤鸣).Biological characteristics and cultivation techniques of Salicornia bigelovii Torr.Modern Veget(当代蔬菜),2006,4:26-27.2 Glenn EP,O'Leary JW,Watson MC,et al.Salicornia bigelovii Torr.:An oilseed halophyte for seawater irrigation.Science,1991,251:1065-1067.3 Zhang L(张莉).Salicornia bigelovii Torr—One crop irrigated by seawater.Trop Agric Guangxi(广西热带农业),2001,4:27-27.4 Qi CH(綦翠华),Zhang W(张伟).The prospects of utilizing halophyte resources in salty environment.Food Nutr China (中国食物与营养).2004,4:25-26.5 Hong LZ(洪立洲),Ding HR(丁海荣),Yang ZQ(杨智青),et al.Research progress and prospects of halopphyte vegetation Salicornia bigelovii Torr.Acta Agric Jiangxi(江西农业学报),2008,20:46-48.6 Li XL(李新丽),Feng YY(冯乙引),Zhao HM(赵厚民).Determination of 7 metal elements from the dry powder of Salicornia bigelovii Torr.by ICP-AES.Chin Wide Plant Resour(中国野生植物资源),2006.25:56-57.7 Zhou F(周峰),Zhou QC(周泉澄),Hua C(华春),et al.Efects of KCI and NaCI on growth and several antioxidative enzyme aetivities of Salicornia bigelovii Torr.Plant Physiol Comm(植物生理学通讯),2008,44:51-54.8 Gu J(顾婕),Yang LP(杨莉萍),Zhao WJ(赵微加),et al.Optimization of extracting procedure of the total flavonoids from Salicornia bigelovii Torr..Li Shizhen Med Mater Med Res(时珍国医国药),2009,20:2274-2275.9 Kong CS,Kim YA,Kim MM,et al.Flavonoid glycosides isolated from Salicornia herbacea inhibit matrix metalloproteinase in HT1080 cells.Toxicol In Vitro,2008,22:1742-1748.10 Geslin M,Verbist JF,Flavonoides de Salicornia europaea.J Nat Prod.1985,48:111-113.11 Kong CS,Kim YA,Kim MM,et al.Antioxidant activity and inhibition of MMP-9 by isorhamnetin and quercetin 3-O-β-D-glucopyranosides isolated from Salicornia herbacea in HT1080 cells.Food Sci Biotechnol,2008,17:983-989.12 Costa CSB,Armstrong R,Detres Y,et al. Effect of ultraviolet-B radiation on salt marsh vegetation:Trends of the genus Salicornia along the Americas.Photochem Photobiol,2006,82:878-886.13 Zhou QC(周泉澄),Hua C(华春).Some physiological response of Salicorni aeuropaea L and Salicornia bigelovii Torr under salt-stress.J Huaiyin Teachers Coll(Nat Sci Ed).(淮阴师范学院学报:自然科学版),2007,6:254-258.14 Ma F(马烽),Zhu YL(朱亚玲),Chen MH(陈明辉),et al.Research progress on extraction process of chiorogenic acid from Flos Lonicerae.Food Res Dev(食品研究与开发),2010,31(7):164-166.15 Zhao Y(赵昱),Zhao J(赵军),Li XP(李湘萍),et al.Advances in caffeoylquinic acid research.Chin J Chin Mater Med(中国中药杂志),2006,31:869-874.16 Wu WH(吴卫华),Kang Z(康桢),Ouyang DS(欧阳冬生),et al.Progress in the pharmacology of chlorogenic acid..Nat Prod Res Dev(天然产物研究与开发),2006,18:691-694.

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海芦笋及其生物盐中多酚酸和绿原酸的定量分析

Quantitative Analysis of Phenolic Acids and Chlorogenic Acid of Salicornia bigelovii Torr.

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