星点设计-效应面法优化杉木枝叶中双黄酮的提取工艺

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (4): 514-519.doi: A

星点设计-效应面法优化杉木枝叶中双黄酮的提取工艺

刘进鹏^1,2，吴纯洁²，辛海量³，张磊⁴，刘品¹，王玉亮^1*

¹上海交通大学农业与生物学院，上海 200240；²成都中医药大学药学院，成都 611137；³第二军医大学中医系，上海 200433；⁴第二军医大学药学院，上海 200433

收稿日期:2010-11-01 出版日期:2012-04-26 发布日期:2012-05-16

Optimum Extracting Process for Biflavone of Leaves and Branches from Cunninghamia lanceolata by Central Composite Design and Response Surface Method

LIU jin-peng^1,2, WU chun-jie², XIN hai-liang³, ZHANG lei⁴, LIU pin¹, WANG yu-liang^1*

¹School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai 200240, China; ²College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; ³Department of Traditional Chinese Medicine, the Second Military Medical University, Shanghai 200433, China;⁴School of Pharmacy, the Second Military Medical University, Shanghai 200433, China

Received:2010-11-01 Online:2012-04-26 Published:2012-05-16

摘要/Abstract

摘要： 采用星点设计-效应面法优化杉木枝叶中穗花杉双黄酮和金松双黄酮的提取工艺。以乙醇体积分数、料液比、提取时间为自变量，穗花杉双黄酮和金松双黄酮转移率的归一化值为因变量，通过对自变量与因变量的二次多项式拟合，采用效应面法选取较优的工艺条件，并进行预测分析。最终确定穗花杉双黄酮和金松双黄酮的最佳提取工艺为：乙醇体积分数为50%，料液比为1:13，超声提取3次，每次提取60 min，最佳工艺验证结果与模型预测值相差–2.55%。结果表明：星点设计-效应面法优选的穗花杉双黄酮和金松双黄酮的提取工艺，方法简便、可靠。

关键词: 杉木, 星点设计, 效应面法, 穗花杉双黄酮, 金松双黄酮

Abstract: The extracting process of leaves and branches of Cunninghamia lanceolata was optimized by central composites design and response surface method. Independent variables were ethanol concentration, solvents ratio and extract time, while dependent variable was OD of extraction rates of Amentoflavone and Sciadopitysin. A two-order polynomial equation was fitted to the data, response surface method was used to optimize the extracting process, and the resultant model was used to predict the response in the optimal region. The optimum extraction conditions for Amentoflavone and Sciadopitysin were obtained as follows: the extracting solvent was 50% ethanol in volume, solid-liquid ratio was 1:13, extracting tine was 60 minutes and extracting thrice. The deviation between observed and predicted values of extraction quantity was –2.55%. The result indicates that the optimum process is simple, convenient and reliable for extracting the leaves and branches of Cunninghamia lanceolata, with a higher precision.

Key words: Cunninghamia lanceolata, central composite design, response surface method, Amentoflavone, Sciadopitysin

中图分类号:

R284.2

刘进鹏，吴纯洁，辛海量，张磊，刘品，王玉亮. 星点设计-效应面法优化杉木枝叶中双黄酮的提取工艺[J]. 天然产物研究与开发, 2012, 23(4): 514-519.

LIU jin-peng, WU chun-jie, XIN hai-liang, ZHANG lei, LIU pin, WANG yu-liang. Optimum Extracting Process for Biflavone of Leaves and Branches from Cunninghamia lanceolata by Central Composite Design and Response Surface Method[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(4): 514-519.

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星点设计-效应面法优化杉木枝叶中双黄酮的提取工艺

Optimum Extracting Process for Biflavone of Leaves and Branches from Cunninghamia lanceolata by Central Composite Design and Response Surface Method

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