北细辛挥发油对二斑叶螨的毒性及其酶的影响

doi:A

天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (4): 525-528.doi: A

北细辛挥发油对二斑叶螨的毒性及其酶的影响

韩俊艳^1,2，王军²，韩雪²，纪明山^1*

¹沈阳农业大学植保学院，沈阳 110866；²沈阳大学生物与环境工程学院，沈阳 110044

收稿日期:2011-02-11 出版日期:2012-04-26 发布日期:2012-05-16
基金资助:
辽宁省科技攻关项目（2008214001），辽宁省高等学校优秀人才支持计划项目（2009R49）

Effects of Essential Oil from Asarum heterotropoides on Toxicity and Related Enzymes of Tetranychus urticae

HAN Jun-yan^1,2, WANG Jun², HAN Xue², JI Ming-shan^1*

¹College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; ²College of Biological and Environmental Engineering, Shenyang University, Shenyang 110044, China

Received:2011-02-11 Online:2012-04-26 Published:2012-05-16

摘要/Abstract

摘要： 采用气相/质谱分析了北细辛挥发油的化学成分，并研究了北细辛挥发油对二斑叶螨的薰蒸毒性，以及对谷胱甘肽巯基转移酶（GSTs）与Na+，K+-ATP 酶活性的影响。结果表明：有9种含量大于1%的化学成分占北细辛挥发油总量的86.1%，其中甲基丁香酚含量最高（22.9%），其次为黄樟醚（19.8%）；北细辛挥发油在8 μg/mL时对二斑叶螨的熏蒸毒性最强，其24和48 h的螨死亡率分别为72.6%和100%；以4 μg/mL北细辛挥发油薰蒸，在24 h内螨Na+，K+-ATP 酶活性下降、GSTs活性升高。本研究结果提示北细辛挥发油有开发为农业用杀螨剂的潜在价值。

关键词: 北细辛, 挥发油, 二斑叶螨, 薰蒸, 谷胱甘肽巯基转移酶

Abstract: To explore new botanical acaricide, the chemical components of Asarum heterotropoides var. mandshuricum essential oils were analysed, and the fumigant toxicity and the effects on related enzymes of the oils to Tetranychus urticae were investigated. In GC-MS analysis, 9 major constituents accounting for 86.1% of the total essential oil, of which methyleugenol (22.9%) was the most, followed by safrole (19.8%). Bioassay showed that the essential oils exhibited the most significant toxicity at 8 μg/mL air concentration with the mortality was 72.6%, 100% and 100% after 24, 48 and 72 h, respectively. In enzyme analysis, 4 μg/mL essential oils decreased the activity of Na+，K+-ATPase, and increased the activity of glutathione S-transferase during 24 h fumigation. As a conclusion, the essential oils of A. heterotropoides possessed the potential for developing botanical acaricide against T. urticae.

Key words: Asarum heterotropoides, essential oils, Tetranychus urticae, fumigant, glutathione S-transferase

中图分类号:

R284.2

韩俊艳，王军，韩雪，纪明山. 北细辛挥发油对二斑叶螨的毒性及其酶的影响[J]. 天然产物研究与开发, 2012, 23(4): 525-528.

HAN Jun-yan, WANG Jun, HAN Xue, JI Ming-shan. Effects of Essential Oil from Asarum heterotropoides on Toxicity and Related Enzymes of Tetranychus urticae[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(4): 525-528.

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北细辛挥发油对二斑叶螨的毒性及其酶的影响

Effects of Essential Oil from Asarum heterotropoides on Toxicity and Related Enzymes of Tetranychus urticae

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