纤维素酶解-超声偶联法提取葛根中总异黄酮的工艺优化

doi:A

摘要/Abstract

摘要： 采用酶解-超声偶联新工艺对葛根中总异黄酮提取进行优化，以提高其收率。在单因素试验的基础上，利用Box-Behnken响应面试验设计，以蒸馏水作为提取溶剂，对温度、酶浓度、时间进行三因素三水平的试验设计优化。结果表明最佳提取工艺条件为：温度34 ℃、酶浓度0.63 mg/mL、时间62 min。在此条件下，葛根总异黄酮提取率为7.10%，与预测值的相对误差为0.34%。酶解-超声偶联工艺是一种新型、快速、有效的总黄酮提取方法。

关键词: 葛根, 总异黄酮, 纤维素酶, 超声辅助, 响应面

Abstract: To increase the extraction yield of total isoflavonoids (TI) from Radix Puerariae, a novel method of cellulase coupling with ultrasound assisted extraction (UAE) was developed. The effects of extraction time, cellulase concentration, and temperature on the yield of TI were studied in single factor experiments. Subsequently, response surface methodology (RSM) was employed to optimize the extraction conditions using distilled water as extraction solvent. A regression model for the yield was established using a Box-Behnken design involving the above three factors at three levels. The experimental results showed the optimum extraction conditions as follows: extraction time of 62 min, enzyme concentration of 0.63 g/mL, temperature of 34 ℃. Under these conditions, the extractions sufficiently reached the maximal TI yield 7.10%. It suggested that the new extraction method is a reliable and potential technology which can be applied in the extraction of TI from Radix Puerariae.

Key words: Radix Puerariae, total isoflavonoids, cellulase, ultrasound, response surface methodology

中图分类号:

R284.2

陈雅维，周惠云，王建普. 纤维素酶解-超声偶联法提取葛根中总异黄酮的工艺优化[J]. 天然产物研究与开发, 2012, 23(7): 933-.

CHEN Ya-wei, ZHOU Hui-yun, WANG Jian-pu. Cellulase Coupling with Ultrasound Assisted Extraction of Total Isoflavonoids from Radix Puerariae[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(7): 933-.

参考文献

1 Cao XL,Tian Y,Zhang TY,et al.Separation and purification of isoflavones from Puerariae lobata by high-speed counter-current chromatography.J Chromatogra.A,1999,855:709-713.
2 Wang CY,Huang HY,Kuo KL,et al.Analysis of puerariae radix and its medicinal preparations by capillary electrophoresis.J Chromatogra A,1998,802:225-231.
3 Yasuda T,Miwa E,Toshiyuki KN,et al.Antipyretic,analgesic and muscle relaxant activities of pueraria isoflavonoids and their metabolites from pueraria lobata Ohwi:a traditional Chinese drug.Biol Pharm Bull, 2005,28:1224-1228.
4 Caia RL,Li M,Xie SH,et al.Antihypertensive effect of total flavone extracts from Puerariae Radix.J Ethnopharmacol,2011,133:177-183.
5 Wong KH,Li G.,Li K M,et al.Kudzu root: traditional uses and potential medicinal benefits in diabetes and cardiovascular diseases.J Ethnopharmacol,2011,134:584-607.
6 Guo ZK,Jin QH,Fan GQ,et al.Microwave-assisted extraction of effective constituents from a Chinese herbal medicine Radix Puerariae.Anal Chim Acta,,2001,436:41-47.
7 Chiu KL,Cheng YC,Chen JH,et al.Supercritical fluids extraction of Ginkgo ginkgolides and flavonoids.J Supercrit Fluids,2002,24:77-87.
8 Lee MH,Lin CC.Comparison of techniques for extraction of isoflavones from the root of Radix Puerariae: Ultrasonic and pressurized solvent extractions.Food Chem,2007,105:223-228.
9 Matthieu V,Valérie T,Carine LB,et al.Towards the industrial production of antioxidants from food processing by-products with ultrasound-assisted extraction.Ultrason Sonochem,2010,17:1066-1074.
10 Zou Y,,Xie CY,Fan GJ,et al.Optimization of ultrasound-assisted extraction of melanin from Auricularia Auricular fruit bodies.Food Sci Emerg Technol,2010,11:611-615.
11 Mostafa K.Optimization of microwave-assisted extraction procedure for zinc and copper determination in food samples by Box-Behnken design.J Food Compos Anal,2009,22:343–346.
12 Ji YL,Hyang SY,Kwang YK,et al.Optimum conditions for the enzymatic hydrolysis of citron waste juice using response surface methodology (RSM).Food Sci Biotechnol.2010,19(5):1135-1142.

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