关键词: 蛹虫草, 酶解, 木瓜蛋白酶, 响应曲面法

Abstract: In order to convert Cordyceps militaris which was a kind of food resouce into a convenient edible form, following experiments were carried out. First, papain was chosen as the enzyme to hydrolyze C. militaris from different kind of proteinase, in which degree of hydrolysis was designed as the target index. Response surface analysis (RSA) was applied to optimize the conditions of enzymatic hydrolysis for C. militaris processing. According to the principle of Box-Behnken central composite design, enzymatic hydrolysis time, enzymeatic hydrolysis temperature, and enzyme dosage were chosen as factors to process the three-factor and three-level central composite design. By response surface analysis, the optimum conditions for enzymatic hydrolysis was obtained as follows: enzymatic hydrolysis time 11.85 h, enzymatic temperature 60.92 ℃, enzyme dosage 1.02%，under which the highest hydrolysis degree reached with estimated value and verified value being 61.27% and 60.76%, respectively. These results demonstrated the validity of this extraction process.

Key words: Cordyceps militaris, hydrolysis, papain, response surface analysis