关键词: 白芍总苷, 组织分布, 冻干粉, 芍药苷, 芍药内酯苷, 高效液相色谱法

Abstract: This paper investigated tissue distribution features of total glucosides of paeony (TGP) in normal rats for providing evidence to predict pharmacologic actions and adverse reaction. Heart, liver, spleen, lung, kidney, stomach, small intestine and large intestine were gotten from normal rats after an oral administration of 2.82 g/kg TGP 1 h, 3 h and 6 h. Homogenate all tissues. Make homogenate into freeze-dry powder, the concentration of paeoniflorin (Pae) and albiflorin (Alb) in which was determined With HPLC. The concentration of the two in all tissues was calculated. 1 h Pae and Alb could be checked out in all tissues. 3 h, except stomach and small intestine, the concentration of Pae and Alb in other tissues arrived the maximum, which was higher in small intestine, stomach, large intestine, kidney, spleen and liver. 6 h the concentration of Pae and Alb in small intestine, large intestine and stomach was higher, other tissues lower. All results demonstrated, after oral administration of TGP, the drug was distributed rapidly and widely in tissues. Small intestine, stomach, large intestine and kidney, spleen, liver were the chief distribution tissues and TGP readily accumulated in stomach and intestines, less in other tissues, which provides guidance for further study of the pharmacological actions and mechanism of TGP, and meanwhile provides some scientific evidences for channel entry theory of white peony root.

Key words: total glucosides of paeony, tissue distribution, freeze-dry powder, paeoniflorin, albiflorin, HPLC