天然产物研究与开发 ›› 2012, Vol. 23 ›› Issue (8): 1089-.doi: A

• 开发研究 • 上一篇    下一篇

酶解大豆蛋白产天冬氨酸蛋白酶抑制剂的研究

张国强,田亚平   

  1. 江南大学工业生物技术教育部重点实验室,无锡214122
  • 出版日期:2012-09-06 发布日期:2012-10-15

The Research of Aspartic Protease Inhibitors from Soy Protein Hydrolyzed by Proteases

ZHANG Guo-qiang,TIAN Ya-ping   

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education Jiangnan University, Wuxi 214122,China
  • Online:2012-09-06 Published:2012-10-15

摘要: 本文通过采用可控酶解的方式酶解大豆蛋白获得了一种源自大豆的天冬氨酸蛋白酶抑制剂SAPI(soy aspartic proteinase inhibitor)。酶解液经过DEAE-52阴离子交换层析、Superdex Peptide 10/300 GL凝胶层析、SOURCE 15RPC反相层析分离后最终比抑制活力为254.2 IU/mg,纯化倍数为62。SAPI对胃蛋白酶(3000U)半数抑制浓度IC50为25.67 μg/mL,有良好的热稳定性,属于一种非竞争性抑制剂。

关键词: 酶解, 大豆蛋白, 天冬氨酸蛋白酶, 抑制剂

Abstract: In this paper, we reported a kind of aspartic protease inhibitor from soy protein (soy aspartic proteinase inhibitor, SAPI) after controlled enzymatic hydrolysis.The final inhibitory activity was 254.2 IU /mg and the purification fold was 62 after DEAE-52 ion-exchange chromatography, Superdex Peptide 10/300 GL gel chromatography and SOURCE 15RPC reversed-phase chromatography. The IC50 value of SAPI was 25.67μg/mL to pepsin(3000U) , SAPI showed good thermostability and belonged to a kind of non-competitive inhibitor.

Key words: enzymolysis, soy protein, aspartic proteinase, inhibitor

中图分类号: 

Q503 Q502 R285