土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用研究

天然产物研究与开发 ›› 2014, Vol. 26 ›› Issue (5): 662-665.

土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用研究

文全泰1，潘廷啟1，黄礼德2，李芸达1，赵善民3*，何显教3，黄彦峰3，晋玲3

1 右江民族医学院临床学院；2 百色市人民医院；3 右江民族医学院生理学教研室，百色 533000

出版日期:2014-05-31 发布日期:2014-11-17

Antioxidant Effect of Talinum crassifolium Extract on D-Galactose Induced Aging Mice

WEN Quan-tai1, PAN Ting-qi1, HUANG Li-de2, LI Yun-da1, ZHAO Shan-min3*, HE Xian-jiao3, HUANG Yan-feng3, JIN Ling3

1 Department of Clinical Laboratory Medicine,Youjiang Medical University for Nationalities; 2 Baise City People's Hospital; 3 Physiology Teaching and Research Section,Youjiang Medical University for Nationalities,Guangxi Baise 533000,China

Online:2014-05-31 Published:2014-11-17

摘要/Abstract

摘要： 本文研究了土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用的影响。取昆明种小白鼠50只，随机分为5组（正常对照组、衰老模型组、土人参提取液低、中、高剂量组）。除正常对照组外，其余各组每天于颈背皮下注射D-半乳糖125 mg/kg，连续42 d，造成亚急性衰老模型。在注射D-半乳糖的同时，三个不同剂量实验组每天分别灌胃土人参提取液5、10和15g/kg，正常对照组及衰老模型组给予等量生理盐水。于42 d后测定小鼠体内超氧化物歧化酶（Super Oxide Dismutase,SOD）、谷胱甘肽过氧化物酶（Glutathione peroxidase,GSH-PX）等酶的活性，及丙二醛（Maleic Dialdehyde,MDA）的含量。结果显示：与正常对照组相比，衰老模型组小鼠心脏、肝脏组织SOD及血清中GSH-PX活性降低（P<0.01），MDA含量增加（P<0.01）。与衰老模型组相比，土人参提取液低、中、高剂量组小鼠心脏、肝脏组织SOD及血清中GSH-PX活性明显升高（P<0.01）；中、高剂量组小鼠心脏、肝脏组织中MDA含量降低明显（P<0.01），低剂量组小鼠肝脏的MDA含量明显降低（P<0.01）。上述结果表明：土人参提取液对D-半乳糖所致衰老小鼠有抗衰老作用。

关键词: 土人参提取液, D-半乳糖, 衰老模型, 抗氧化作用

Abstract: In the present study,the effect of Talinum crassifolium Extract on antioxidation in aged mice induced by D-galactose was investigated.Fifty male Kunming mice were randomly divided into five groups,namely control group,model group,and T.crassifolium low,medium and high-dose groups.Except the control group,all other groups were given with 125 mg/kg of D-galactose by subcutaneous injection at neck nape for 42 d.At the same time,mice in the three sample groups were gavaged with 5,10 and 15 mg/kg of T.crassifolium extract;and mice in control group and model group were gavaged with the same volume of normal saline.The activities of Super Oxide Dismutase (SOD),Glutathione peroxidase (GSH-PX),and the content of Maleic Dialdehyde (MDA) in mice were then determined.The resulted showed that the levels of GSH-PX in blood serum and SOD in liver,heart of the model group were significantly lower than these of normal control group (P<0.01).The activities of MDA in liver and heart of the model group significantly increased compared with these of normal control group (P<0.01).The levels of GSH-PX in blood serum and SOD in liver,heart of T.crassifolium low, medium and high-dose groups were significantly higher than these of model groups (P<0.01).The levels of MDA in liver and heart of T.crassifolium medium and high doses groups were significantly lower than these of model groups (P<0.01).The of activity of MDA in liver of T.crassifolium low dose group was significantly lower than that of normal control group (P<0.01).Based on these results,it was concluded that T.crassifolium extract had antiaging effect on D-galactose induced aging mice.

Key words: Talinum crassifolium extract, D-galactose, aging model, antioxidant

中图分类号:

R965

文全泰1，潘廷啟1，黄礼德2，李芸达1，赵善民3*，何显教3，黄彦峰3，晋玲3. 土人参提取液对D-半乳糖所致衰老小鼠的抗氧化作用研究[J]. 天然产物研究与开发, 2014, 26(5): 662-665.

WEN Quan-tai1, PAN Ting-qi1, HUANG Li-de2, LI Yun-da1, ZHAO Shan-min3*, HE Xian-jiao3, HUANG Yan-feng3, JIN Ling3. Antioxidant Effect of Talinum crassifolium Extract on D-Galactose Induced Aging Mice[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2014, 26(5): 662-665.

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