关键词: 总黄酮, 比色法, 五脉绿绒蒿, 全缘绿绒蒿, 红花绿绒蒿, 总状绿绒蒿

Abstract: In this paper,the contents of total flavonoids in four Meconopsis species collected from the Qinghai-Tibet plateau were determined by ultraviolet-visible spectrophotometer with colorimetric method.This method showed good linearity (r²=0.9990).The average recovery of the method was 101.56%;the accuracy and repeatability of the method were confirmed by the precision and repetition tests with RSD=2.94% and 1.34%,respectively.The results showed that the total flavonoids content in Meconopsis quintuplinervia was the highest,which was a top-grade medicinal herb in accord with the traditional usage habit of Tibet.In addition,the results showed that there was a remarkable difference between Meconopsis quintuplinervia and the others which was analyzed by SPSS 20.0 with ANOVA method.The results presented in this study,will provide a theoretical basis for the further studies of the medicinal herbs of Meconopsis species.

Key words: total flavonoids;colorimetric method;Meconopsis quintuplinervia, Meconopsis integrifolia, Meconopsis horridula, Meconopsis punicea