天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (6): 961-967.doi: 10.16333/j.1001-6880.2020.6.008

• 研究论文 • 上一篇    下一篇

酸枣叶醇提物对小鼠巨噬细胞M1和M2型极化的影响

董建新1，刘晓光1，李晨曦1，祁雪慧1，朱毓永1，王悦2，单梅华1*   

  1. 1河北民族师范学院 生物与食品科学学院，承德 067000；2南开大学医学院，天津 300071
  • 出版日期:2020-06-28 发布日期:2020-07-06
  • 基金资助:
    陕西省肿瘤精准医学重点实验室开放课题（KLTPM-SX2019-A3）；河北省高等学校科学技术研究资助项目（QN2019301）；承德市基础研究项目（201903A047）；河北民族师范学院塞罕坝专项（ZD2017004）

Effect of jujube leaves alcohol extract on M1 and M2 polarization of mouse macrophages 

DONG Jian-xin1,LIU Xiao-guang1,LI Chen-xi1,QI Xue-hui1,ZHU Yu-yong1,WANG Yue2,SHAN Mei-hua1*   

  1. 1Department of Biology and Food Science,Hebei Normal University for Nationalities,Chengde 067000,China;2School of Medicine,Nankai University,Tianjin 300071,China
  • Online:2020-06-28 Published:2020-07-06

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摘要：

为探究酸枣叶醇提物（jujube leaves alcohol extract，JLE）对RAW264.7细胞M1和M2型极化的影响。本研究分别采用LPS和IL-4将RAW264.7细胞诱导极化为M1和M2型巨噬细胞，CCK8方法检测细胞存活率，Griess法检测药物对M1型细胞释放NO的影响，RT-PCR法检测药物对M1和M2型细胞标志基因表达的影响，划痕实验和RT-PCR法检测经药物处理的M2型巨噬细胞上清对4T1细胞迁移的影响。结果显示，酸枣叶醇提物可抑制NO的释放，下调M1型IL-12p40、IL-1β、COX-2和M2型Arg1、CD206的表达。并通过M2型巨噬细胞降低4T1细胞中MMP-2、MMP-9和NF-κBp65的mRNA水平，抑制4T1细胞的迁移。综上说明酸枣叶醇提物可抑制巨噬细胞M1和M2型极化，并通过NF-κB通路抑制4T1细胞迁移。

关键词: 酸枣叶醇提物, RAW264.7细胞, M1和M2型极化, 细胞迁移

Abstract:

To explore the effect of jujube leaves alcohol extract (JLE) on M1 and M2 polarization of RAW264.7 cells.In this study,RAW264.7 cells were polarized into M1 and M2 macrophages by LPS or IL-4,respectively.CCK8 was used to detect the cell survival rate.Griess method was used to detect the effect of drugs on the release of NO in M1 macrophages.RT-PCR was used to detect the expression of M1 and M2 marker genes.Scratch test and RT-PCR were used to detect the migration of 4T1 cells treated by M2 macrophages culture supernatant.The results showed that the jujube leaves alcohol extract could inhibit the release of NO,down-regulate IL-12p40,IL-1β and COX-2 in M1 macropage and Arg1,CD206 in M2 macrophage.Further,it could reduce the mRNA level of MMP-2,MMP-9 and NF-κBp65 in 4T1 under the influence of M2 macrophages,and inhibit the migration of 4T1 cells.In conclusion,the jujube leaves alcohol extract can inhibit M1/M2 polarization of macrophages,and inhibit the migration of 4T1 cells through NF-κB pathway.

Key words: jujube leaves alcohol extract, RAW264.7 cells, M1 and M2 polarization, cell migration

中图分类号:  R966 R285

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