天然产物研究与开发 ›› 2020, Vol. 32 ›› Issue (8): 1405-1412.doi: 10.16333/j.1001-6880.2020.8.017

• 开发研究 • 上一篇    下一篇

壳聚糖生姜精油涂膜对鲜银耳活性氧代谢和组织超微结构的影响

罗杨1,3,李娟1,陈岗1,2,吴振1,3,詹永1,2,廖霞1,杨勇1,2*   

  1. 1重庆市中药研究院;2重庆市中药大健康工程技术研究中心;3中药健康学重庆市重点实验室,重庆 400065

  • 出版日期:2020-08-28 发布日期:2020-09-02
  • 基金资助:
    重庆市技术创新与应用示范(社会民生类)项目(cstc20 18jscx-mszdX0046,cstc2018jscx-msybX0209);重庆市科技局鲁渝科技协作项目(cstc2019jscx-lyzxX0002)

Effect of chitosan ginger oil coating on ROS metabolism and microstructure of fresh tremella

LUO Yang1,3,LI Juan1,CHEN Gang1,2,WU Zheng1,3,ZHAN Yong1,2,LIAO Xiao1,YANG Yong1,2*#br#

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  1. 1Chongqing Academy of Chinese Materia Medica;2Chongqing Engineering Technology Research Center of Materia Medica Health; 3Chongqing Key Laboratory of Chinese Medicine & Health Science,Chongqing 400065,China

  • Online:2020-08-28 Published:2020-09-02

摘要: 为探讨壳聚糖生姜精油涂膜保鲜处理对采后鲜银耳活性氧代谢的影响,用浓度为1.0%壳聚糖-生姜精油处理新鲜银耳,测定其对鲜银耳抗病性及活性氧代谢的影响。结果表明,壳聚糖生姜精油处理显著降低鲜银耳发病率及病情指数(P<0.05);涂膜处理可在贮藏前期促进O2含量升高,并提高PPO、CAT和SOD活性,抑制POD活性。说明壳聚糖-生姜精油涂膜处理可以诱导银耳体内活性氧爆发,加速银耳体内活性氧清除反应,增强银耳抗病性,延缓鲜银耳采后成熟衰老。超微结构观察表明,壳聚糖-生姜精油成膜液形成致密的网状结构,附着在银耳表面,填充了银耳菌丝细胞空隙,隔绝外界微生物的侵染,防止鲜银耳水分及营养成分流失。


关键词: 壳聚糖, 生姜精油, 鲜银耳, 涂膜保鲜, 活性氧代谢

Abstract:

The effect of chitosan ginger essential oil coating preservation treatment on active oxygen metabolism of fresh tremella was investigated. The concentration of 1.0% chitosan ginger essential oil was used to treat fresh tremella,and the effects of chitosan ginger essential oil on disease resistance and active oxygen metabolism of fresh tremella were determined. The results showed that chitosan ginger essential oil coating treatment significantly reduced the disease incidence and index of fresh tremella (P<0.05). In the early stage of storage,the contents of O2,the activities of PPO,CATand SODwere increased,and the POD activity was inhibited. The results showed that chitosan ginger oil coating treatment could induce reactive oxygen species (ROS) and accelerate ROS scavenging reaction intremella,also enhance disease resistance,delay the maturity and senescence of fresh tremella. The ultrastructure observation showed that the chitosane-ginger essential oil membrane forming liquid formed a dense network structure,adhered to the surface of tremella,filled the space of tremella mycelium cells,isolated the infection of external microorganisms,and prevented the loss of water and nutrients of fresh tremella.

Key words: chitosan, ginger oils, fresh tremella, coating preservation, reactive oxygen species metabolism

中图分类号:  TS255.3