天然产物研究与开发 ›› 2021, Vol. 33 ›› Issue (9): 1548-1557.doi: 10.16333/j.1001-6880.2021.9.013

• 开发研究 • 上一篇    下一篇

不同剂量解钾菌对滇重楼生长发育的影响

周浓1,许凌峰1,杨敏2,郭冬琴1,丁博1,朱芙蓉1,赵晶晶1*   

  1. 1重庆三峡学院生物与食品工程学院,重庆 404000;2大理大学药学与化学学院,大理
  • 出版日期:2021-09-28 发布日期:2021-09-28
  • 基金资助:
    国家自然科学基金(81260622);重庆市自然科学基金(cstc2018jcyjAX0267);云南省地方本科高校(部分)基础研究联合专项(2019FH001(-041));“成渝地区双城经济圈建设”科技创新项目(KJCX2020046)

Effect of different dosage of potassium-dissolving bacteria on growth and development of Paris polyphylla var. yunnanensis

ZHOU Nong1,XU Ling-feng1,YANG Min2, GUO Dong-qin1,DING Bo1,ZHU Fu-rong1,ZHAO Jing-jing1*#br#

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  1. 1College of Life Science and Engineering,Chongqing Three Gorges University,Chongqing 404000,China;2College of Pharmacy and Chemistry,Dali University,Dali 671000,China

  • Online:2021-09-28 Published:2021-09-28

摘要:

为深入研究不同剂量的解钾菌对滇重楼生长发育的影响。本研究通过盆栽试验,于滇重楼苗期采用灌根方式接种解钾菌,接种剂量依次为5.83、17.50和35.00 g,施用等量清水为对照(CK)。结果表明,与CK相比,滇重楼苗期接种解钾菌可提高根系活力,增加叶片内叶绿素、类胡萝卜素、可溶性蛋白和可溶性糖含量,降低叶片内保护酶(超氧化物歧化酶、过氧化物酶和过氧化氢酶)活性以及丙二醛含量;接种解钾菌植株的老根茎和新根茎内营养元素锰(Mn)、镁(Mg)和锌(Zn)含量有所增加,而重金属铅(Pb)、铜(Cu)、砷(As)和镉(Cd)含量普遍降低。可见,不同时期接种解钾菌均可促进滇重楼植株的生长发育,增加其根茎内有益营养元素的积累,降低重金属元素含量。其中,接种35.00 g解钾菌对滇重楼幼苗生长的促进效果最佳,17.50 g解钾菌的调控效果次之。

关键词: 滇重楼, 解钾菌, 生长发育, 重金属

Abstract:

Effect of different doses of potassium-dissolving bacteria on growth and development in Paris polyphylla var. yunnanensis was researched.In this study,the pot inoculation experiment was conducted to inoculate potassium-dissolving bacteria during the seedling stage of Paris polyphylla var. yunnanensis.The inoculated doses of potassium-dissolving bacteria were 5.83,17.50 and 35.00 g,and the same amount of water was used as the control (CK).The result showed that root vigor,chlorophyll,carotenoids,soluble protein and soluble sugar contents were increased,protective enzyme (superoxide dismutase,peroxidase and catalase) activities and malondialdehyde content were reduced by inoculation of potassium-dissolving bacteria,compared with CK.The nutrient element in new rhizomes and old rhizomes of the plants inoculated with potassium-dissolving bacteria were increased,such as manganese (Mn),magnesium (Mg) and zinc (Zn),while heavy metals were generally decreased including plumbum (Pb),cuprum (Cu),arsenic (As) and cadmium (Cd).The results showed that the seedling stage of Paris polyphylla var. yunnanensis inoculation with potassium-dissolving bacteria can promote the growth and development,promote the accumulation of nutrient elements in its rhizomes,and reduce contents of heavy metals.Among them,inoculation with 35.00 g of potassium-dissolving bacteria had the best effect on promoting the growth of Paris polyphylla var. yunnanensis,and followed by 17.50 g of potassium-dissolving bacteria.

Key words: Paris polyphylla var. yunnanensis, potassium-dissolving bacteria, growth and development, heavy metal

中图分类号:  R282.2