天然产物研究与开发 ›› 2011, Vol. 23 ›› Issue (6): 1088-1090.doi: A

• 研究简报 • 上一篇    下一篇

温度诱导双水相体系分离芦荟多糖的分配行为研究

李文君,李芬芳*,邢健敏   

  1. 中南大学化学化工学院,长沙410083
  • 收稿日期:2010-05-26 出版日期:2011-12-22 发布日期:2012-07-04
  • 基金资助:

    国家自然基金项目(20956001);湖南省重点基金项目(08JJ3023)

Partitioning Behaviour of Aloe Polysaccharides by Temperature-Induced Aqueous Two-Phase System

LI Wen-jun, LI Fen-fang*, XING Jian-min   

  1. School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
  • Received:2010-05-26 Online:2011-12-22 Published:2012-07-04

摘要: 本文研究了芦荟多糖在温度诱导双水相体系中的分配行为,考察了Trition-114的浓度、温度、酸度、盐的浓度等因素对芦荟多糖分配行为的影响。结果表明,芦荟多糖趋于分配在水相,当Trition-114浓度为4%,pH=3,温度50℃时,芦荟多糖在水相中的回收率达到最大,多糖的含量也由原来的68.39%增加到75.63%。实验还表明,无机盐对芦荟多糖的分配行为具有很大的影响。

关键词: 温度诱导, 芦荟多糖, 双水相

Abstract: In this paper, the partitioning behavior of Aloe polysaccharides was investigated with TritonX-114 temperature-induced aqueous two-phase system. The effects of different factors including the concentration of Triton X-114, temperature, pH, and concentration of inorganic salt were investigated in details. The results showed that Aloe polysaccharides mostly accumulated in the aqueous phase. The optimal condition consisted of adding 4% (w/w) Triton X-114 and 0.3 mol/L inorganic salt, pH 3.0 and 50 ℃ under which the highest recovery rate was obtained and the content of Aloe polysaccharides increased from 68.39% to 75.63%. In addition, it was demonstrated that the inorganic salt had a great effect on the partitioning behavior of Aloe polysaccharides.

Key words: temperature-induced, aloe polysaccharides, aqueous two-phase

中图分类号: 

R284.2