Abstract: To isolate fibrinolytic active compounds from cultures of marine fungi FG216 and initial identify of the strain, czapek medium was choosed as fermentation medium. The active compounds with fibrinolytic activity measured by microplate reader were isolated from a culture broth and refined with marine fungi FG216 by using semi-preparative HPLC. In the reciprocal activation system of prourokinase and plasminogen, the activity was highest when the additive amount of fibrinolytic active compound was 10 μg/mL. The complete ITS sequence of FG216 strain was cloned and sequenced, and phylogenetic tree based on ITS-5.8 S rDNA sequences showed that FG216 strain has a high homology with Stachybotrys longispora on taxonomic level. Reciprocal activation of prourokinase and plasminogen was enhanced by the fibrinolytic active compound with the strain of marine fungi FG216.

Key words: fibrinolytic active compound, isolation, HPLC, strain identification, Stachybotrys