The Research of Aspartic Protease Inhibitors from Soy Protein Hydrolyzed by Proteases

doi:A

Abstract

Abstract: In this paper, we reported a kind of aspartic protease inhibitor from soy protein (soy aspartic proteinase inhibitor, SAPI) after controlled enzymatic hydrolysis.The final inhibitory activity was 254.2 IU /mg and the purification fold was 62 after DEAE-52 ion-exchange chromatography, Superdex Peptide 10/300 GL gel chromatography and SOURCE 15RPC reversed-phase chromatography. The IC50 value of SAPI was 25.67μg/mL to pepsin(3000U) , SAPI showed good thermostability and belonged to a kind of non-competitive inhibitor.

Key words: enzymolysis, soy protein, aspartic proteinase, inhibitor

CLC Number:

ZHANG Guo-qiang，TIAN Ya-ping. The Research of Aspartic Protease Inhibitors from Soy Protein Hydrolyzed by Proteases[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2012, 23(8): 1089-.

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