Abstract: Five nucleosides(adenosine,cordycepin,uridine,inosine and guanosine)in three cordyceps(Cordyceps jiangxines, Cordyceps gunni, Cordyceps taii) were quantified using a developed high-performance liquid chromatography-diode array detector(HPLC-DAD)method.The mycelium and fermentation broth was obtained by the submerged cultivation.The nucleosides compoundswas extracted through ultrasonic fracture method.HPLC-DAD was applied for the quantitative analysis with retention time andabsorption spectrum adopted as qualitative evidences.The chromatographic separation conditions were optimized.The precision, stability, reproducibility and recovery were good which indicated the developed HPLC-DAD was valid for the quantification ofnucleosides.This was the first report of determining nucleosides contents in both intracellular and extracellular parts of three cordyceps.

Key words: Cordyceps, cultivation, mycelium, nucleosides, high-performance liquid chromatography, quantitative determination