Abstract: A new ginsenoside Rg1-bonded silica gel stationary phase (250 mm×4.6 mm,5 μm) was prepared by one-pot reaction methodusing 1,6-diisocyanate as spacer arm.The characterization of the new self-made ginsenoside Rg₁-bonded silica gel stationary was done by elemental analysis,thermal analysis and scanning electron microscope(SEM).The HPLC fingerprint of Active Pharmaceutical Ingredient (API) of Panax notoginseng saponins (PNS) on the ginsenoside Rg₁-bonded silica gel stationary phase was established.The chromatographic fingerprints of 10 batches PNS API were determined on the self-made ginsenoside Rg1-bonded silica gel column.The mobile phase consisted of acetonitrile and water with gradient elution mode.The detection wavelength was 203 nm,the column oven temperature was maintained at 25 ℃,the flow rate was 0.8 mL/min and the injection volume was 10 μL.Based on the peak of ginsenoside Re,20 common peaks were selected,the similarity between 10 batches of samples were within 0.882-0.999.This study provided a new idea for natural products analysis using ginsenoside Rg1-bonded silica gel stationary phase.

Key words: HPLC, fingerprint, ginsenoside Rg1-bonded silica gel stationary phase, characterization of stationary phase, active pharmaceutical ingredient of Panax notoginseng saponins