Abstract: The aim of this study was to investigate the preparation process of vaccarin ointment and its stability.Multifactor ratio experiment with the emulsion process was adopted to prepare ointment.The content determination was carried out by HPLC method.The optimal formulation contained 15% (mass fraction) stearic acid glyceride,20% liquid paraffin wax,2% stearic acid,6% octadecanol,25% pure glycerin,30% distilled water,2% sodium dodecyl sulfate,0.1% hydroxy ethyl benzene and 0.1%vaccarin.The optimized process was simple and feasible.The quality of vaccarin ointment prepared using the optimized process was stable and controllable.

Key words: vaccarin, ointment, preparation, high Performance liquid chromatography