Abstract: In this study,the anti-aging effect of MSMI (melanin from Sepiella maindroni ink) on mimetic aging mice induced by D-galactose was investigated.ICR mice was selected as animal model,which were randomly divided into six groups,including control group,D-galactose group,positive control group (Vc group),MSMI treated groups of low ［25 mg/(kg·d)］,medium ［120 mg/(kg·d)］ and high ［200 mg/(kg·d)］ dosage.Except control group,all the other groups were treated with 120 mg/(kg·d) D-galactose by subcutaneous injection.After a trial period of 45 days,the content of MDA (malondialdehyde) and the activities of CAT (catalase),SOD (superoxide dismutase) and GSH-Px (glutathione peroxidase) in serum,liver,brain and heart were measured.Besides,the liver and thymus index were measured.The results showed that compared with D-galactose group,MSMI can significantly decrease the contents of MDA and enhance the activities of CAT,SOD,GSH-Px in serum,liver,brain and heart of mice.Hence,it was concluded that MSMI can attenuate the increased oxidative damages in D-galactoseinduced senescent mice and delay aging.

Key words: melanin, Sepiella maindroni, antioxidation, D-galactose