Abstract: To establish a method for the simultaneous determination of 4 flavonoids in Radix Hedysari.Using Agilent HCC18(4.6 mm × 250 mm,5 μm),column temperature of 25 ℃,flow rate of 1.0 mL/min;the detection wavelength were 248 nm,258 nm;mobile phase:0.3% aqueous formic acid(A)acetonitrile(B);gradient elution program for the 012 min,20%37%B;1216 min,37%40%B;1622 min,40%50%B;2228 min,50%100%B.With formononetin as internal standard,the relative correction factor to establish formononetin and calycosin glycoside,ononin,calycosin,and use the correction factor of calycosin glycoside,ononin,calycosin content was calculated,the calculated value,realize QAMs method,at the same time,using external standard method for the determination of calycosin glycoside herbs,ononin,calycosin,formononetin content,so the measured value.Then compare the difference between measured value and calculated value.Use a test determination of multi components by the 12 batch of 4 flavonoids in Radix Hedysari contents,establishing the correction factor and good reproducibility,content using correction factors calculated values showed no significant difference between the measured value and the external standard method.The external standard method for the determination of formononetin,using the relative correction factor of realization of formononetin and calycosin glycoside,ononin,calycosin determination is accurate and feasible.

Key words: Radix Hedysari, QAMs, high performance liquid chromatography, flavonoids