Abstract: The objective of this experiment was to study the effects of honeybee comb extract on immune regulation in mice. 160 mice were randomly divided into four batches,each batch contained four groups:negative control group,low dose,medium dose and high dose honeybee comb extract groups. The body weight,index of immuneorgans,indexes of spleen lymphocyte transformation,hemolysin value of blood serum,NK cell activity and antibody cell number of spleen were then screened. The results showed that no significant difference of body weight of mice was detected between the negative control group and experimental groups. Ratio of each viscera weight/body weight in mice,the thymus weight/body weight ratio also had no obvious change. In addition,the high and medium dose group of honeybee comb extracts greatly improved hemolysin value of blood serum and indexes of spleen lymphocyte transformation (P<0.05). Compared with negative control group,three experimental groups significantly improved the activity of NK cells in mice (P<0.05). Hence,it was concluded that honeybee comb extract had a promoting effect on immune functions of mice.

Key words: honeybee comb, mice, immune regulation, cellular immunity, humoral immunity