Abstract: The goal of the present study was to improve the production of HMG-CoA reductase inhibitors MFS (Metabolite of Fusarium solani FG319),through optimizing the fermentation mediums and conditions of marine microorganism Fusarium solani FG319.GSB medium,CYM medium,ATCC medium,BPY medium,TYG medium,Oatmeal medium,Czapek-Dox (CD) medium and potato dextrose broth culture medium were used to optimize the fermentation conditions by shake flask fermentation and fermenter.The production of MFS was analyzed using a HPLC by chromogenic substrate.In addition,an inhibitory effect of MFS on HMG-CoA reductase was determined in vitro. The results showed that CD medium was the best medium for marine microorganism Fusarium solani FG319;maximum production of HMG-CoA reductase inhibitor MFS was achieved on 7^th day;inducer L-ornithine hydrochloride had increased the production of MFS (4.07 times);in all sugar type,the marine microorganism Fusarium solani FG319 metabolic MFS quantity was up to 23.47 mg/L without NaCl (pH = 6.9); in vitro evaluation system showed inhibition activity of MFS on HMG-CoA reductase was higher than the activity of lovastatin (1.38 times) and pravastatin (1.74 times).The medium selection and optimization of fermentation conditions significantly improved the production of HMG-CoA reductase inhibitor from marine microorganism Fusarium solani FG319.

Key words: optimization, culture conditions, Fusarium solani FG319, HMG-CoA reductase