Abstract: To clarify chemical constituents in discharged wastewater of paper-making reconstituted tobacco,the discharged wastewater was extracted by ethyl alcohol after electrodialysis desalination and concentration.The extracts were purified by chromatographic techniques,which led to the isolation of four compounds,including one new sesquiterpenoid nicotianolide (1),one known sesquiterpenoid 2β-hydroxysolanascone (2),and two known alkaloids 5,6dihydro-2(1H)-pyridinone (3) and cotinine (4).Their structures were elucidated by means of spectroscopic methods including 1D and 2D nuclear magnetic resonance (NMR) and mass (MS) spectra.

Key words: Tobacco, wastewater, sesquiterpenoids, alkaloids