Abstract: To study the protection of Rosemary Extract (RE) on acute alcoholic liver injury in mice,and research the mechanism from alcohol metabolism,lipid metabolism,anti-oxidative and anti-inflammation.Mice were randomly divided into control group,model group,Metadoxine Capsules group (MC,200mg/kg·d),RE group (80,160,400 mg/kg·d).The mice were given drugs by gavage 1 time a day and for 30 days.Then,acute alcoholic liver injury model was established by intragastric administration of 56% alcohol (13mg/kg) to the medication and model groups 1h later after the last administration.12h later,the levels of serum ALT,AST,TG,TNF-α,LI-6,IL-10 were detected.The contents of hepatic MDA,SOD,GSH-Px,ADH were determined.The mRNA levels of FAS,ADRP,CYP2E1,PPARα and Caspase3 in liver were also measured by real time PCR.HE straining was performed for observing pathological changes of the liver tissues.Compare with model group,RE can reduce the activity of ADH and contents of ALT,AST,and TG in serum,and shorten the sober time,down regulate Caspase3 expression and relieve acute alcohol liver tissue lesions.The protection mechanism research found thatRE can suppress acute ALD by down-regulatingFAS and ADRP,which can reduce the TG concentration in serum.RE also cure acute ALD by enhancing the activity of SOD,GSH-Px and expressing of PPARα,and down-regulating the expression of CYP2E1,to reduce the level of MDA in liver and relieve oxidative damage.RE can reduce the hepatic inflammation by decreasingTNF-α,IL-6 and increasing IL-10.These results showed thatRE can protect acute alcoholic liver injury by antioxidative,anti-inflammation and regulating lipid metabolism.

Key words: rosemary extract, acute alcoholic liver injury, oxidative damage, lipid metabolism, inflammation