Relationship Between HPLC Fingerprint Chromatogram and Antioxidant Activity of Forsythia suspense Leaves

doi:10.16333/j.1001-6880.2017.4.017

Abstract

Abstract: A spectrum effect correlation pattern was developed to evaluate the antioxidant activity and the main active components of Forsythia suspense leaves in this study.The fingerprint chromatogram of the 18 Forsythia suspense leaves extracts collected from different places was established by HPLC.In addition the antioxidant activity and the content of total phenol of these 18 extracts were studied using the 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·)-scavenging method and Folin-Ciocalteuas method,respectively.The results showed that the antioxidant activity was correlated to the content of total phenol by the analysis of bivariate correlation.Furthermore,three components played a role in antioxidant activity of F.suspense leaves through the analysis of principal component,bivariate correlation and multiple linear regression.The study provided reference for the evaluation of antioxidant activity of F.suspense leaves.

Key words: Forsythia suspense leaves, fingerprint, antioxidant activity, spectrum-effect correlation pattern

CLC Number:

R284.2

ZHANG Yuan-bo1,2,ZHANG Min3, CHENG Qi-bin1,MA Li-sha1,ZHANG Li-wei1*. Relationship Between HPLC Fingerprint Chromatogram and Antioxidant Activity of Forsythia suspense Leaves[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(4): 629-634.

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Relationship Between HPLC Fingerprint Chromatogram and Antioxidant Activity of Forsythia suspense Leaves

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