Relationship Between HPLC Fingerprint Chromatogram and Antioxidant Activity of Forsythia suspense Leaves

doi:10.16333/j.1001-6880.2017.4.017

Abstract

Abstract: A spectrum effect correlation pattern was developed to evaluate the antioxidant activity and the main active components of Forsythia suspense leaves in this study.The fingerprint chromatogram of the 18 Forsythia suspense leaves extracts collected from different places was established by HPLC.In addition the antioxidant activity and the content of total phenol of these 18 extracts were studied using the 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·)-scavenging method and Folin-Ciocalteuas method,respectively.The results showed that the antioxidant activity was correlated to the content of total phenol by the analysis of bivariate correlation.Furthermore,three components played a role in antioxidant activity of F.suspense leaves through the analysis of principal component,bivariate correlation and multiple linear regression.The study provided reference for the evaluation of antioxidant activity of F.suspense leaves.

Key words: Forsythia suspense leaves, fingerprint, antioxidant activity, spectrum-effect correlation pattern

CLC Number:

R284.2

ZHANG Yuan-bo1,2,ZHANG Min3, CHENG Qi-bin1,MA Li-sha1,ZHANG Li-wei1*. Relationship Between HPLC Fingerprint Chromatogram and Antioxidant Activity of Forsythia suspense Leaves[J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2017, 29(4): 629-634.

Add to citation manager EndNote|Ris|BibTeX

URL: http://www.trcw.ac.cn/EN/10.16333/j.1001-6880.2017.4.017

http://www.trcw.ac.cn/EN/Y2017/V29/I4/629

[1]	CUI Hao-lin, CHEN Jia-qi, GUO Yong-fa, ZHOU Jia-liang, JING Cheng, ZHOU Xin-jie, YIN Shuai-wen. Optimization of ultrasonic-assisted extraction flavonoids from Cardiocrinum cathayanum bulbs by response surface methodology and its bioactivity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(5): 978-988.
[2]	JIANG Kai-hang, ZHANG Ming-tong, DONG Yang-li, LIU Zhi-hao, LIU Dong-sheng, ZHANG Sheng-jie, LI Dong-hua, MA Xiao. Chemical composition differences between Rubiae Radix et Rhizoma and its adulterants Based on HPLC fingerprint and chemical pattern recognition combined with multi-component quantification [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(4): 701-714.
[3]	ZHOU Lin, LIU Xiao-xia, XIE Wen-kai, DUAN Zhi-wen, FENG Yong-wei, LI Zhen-yu, ZHANG Jun, CHEN Xiang-dong. Quality evaluation of Morindae Officinalis Radix and its processed products based on HPLC-ELSD fingerprint and multi-component quantification combined with chemometrics [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(3): 509-520.
[4]	WANG Xiu, CHEN Jing-hua, LI Qi-yu, WANG Miao-miao, YU Rui-tao. Isolation,identification and antioxidant activity of a new flavonol tetraglycoside from Lepidium latifolium L. [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(3): 521-528.
[5]	ZHANG Fang, ZHAO Juan. Optimization of microwave-assisted alcohol extraction process and in vitro antioxidant activity of flavonoids from Astragali Radix [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(3): 556-563.
[6]	DU Zhong-hua, XU Lei, HU Zhi-mei, ZHANG Ming, XIA Heng-jian, WANG Sheng-jun, YE Li-chun, SHI Zhao-hua. Quantitative value transfer of key substances at different stages of the preparation process of Prunellae Spica ointment [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(2): 368-383.
[7]	GAN Ping-gui, DENG Ye-cheng, DENG Zhi-yong, LUO Hai-yu. In vitro antioxidant activity of hydrolysates of fly maggots [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(2): 384-392.
[8]	PENG Zhi-cheng, YANG Jie, LIANG Yue-yi, HOU Xu-xuan, LI Zhen-yu, LYU Wei-sheng, CHEN Rou-shan. Study on the quality marker of Lycii Cortex in the treatment of "three hypers" based on fingerprint,network pharmacology and quantitative analysis [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(2): 425-434.
[9]	TANG Lin, LU Ai-juan, HU Chao, LI Hai-ying, GAO Yuan-hang, LI Hao, YANG Lei. Spectrum-effect relationship between HPLC fingerprint and in vitro antioxidant activity of Notoginseng Radix et Rhizoma and its processed products [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2026, 38(1): 1-12.
[10]	LIU Jin-zhen, JIANG Rong-yue, WEI Qing, WANG Hai-jiao, WANG Zhi-wei, HAN Hui-pei, MA Xiao-jing. Comparative study on the constituents and biological activities of different basil essential oils [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(增刊1): 68-75.
[11]	LI Yong-dong, HUO Qin-jian, YANG Ming-hua, LI Yun-qiang, JIN Hui, YANG Min-min, LIAN Yun, LIU Jin-liang. Study on browning marker of tobacco leaf based on HPLC fingerprint and antioxidant spectrum-effect relationship [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(增刊1): 99-106.
[12]	ZENG Zi-hang, PEI Chao-yang, WU Meng-mei, CAO Hui, ZHANG Ying, WU Meng-hua, MA Zhi-guo. Comparative study on HPLC fingerprints and four constituents content of Atractylodis Macrocephalae Rhizoma before and after processing with rice-washed water [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(9): 1595-1604.
[13]	SUN Ying-di, ZHONG Ai-yu, ZHANG Chen-yang, ZHAO Li-yan, ZHANG Wan-ming, ZHANG Yuan-yuan. Establishment of UPLC fingerprint of Sophorae Flavescentis Radix and its anti-tumor spectral effect relationship [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(8): 1460-1470.
[14]	YANG Niu-niu , XIE Zhi-feng, LYU Dan, LI Jing-yue, WANG Shu-xian, LI Jiao, LI Cong-hu, CHENG Xu. Optimization of ultrasound-assisted deep eutectic solvent extraction and the antioxidant activity of essential oil from Trichosanthes kirilowii seeds [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(7): 1310-1321.
[15]	RU Xin-xin, ZHAO Yi-yang, ZHANG Wan-zhong, ZHU Jian-xing. Optimization of microwave-assisted cellulase extraction of polyphenols from Moringa oleifera seeds and its activity [J]. NATURAL PRODUCT RESEARCH AND DEVELOPMENT, 2025, 37(7): 1322-1330.

Relationship Between HPLC Fingerprint Chromatogram and Antioxidant Activity of Forsythia suspense Leaves

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments