Abstract: In this study,15 compounds were isolated and purified from the 95% EtOH extract from Hypoestes triflora by column chromatography on silica gel,Sephadex LH-20 and HPLC.Their structures were elucidated as 6-hydroxy-9,13-cyclomegastigma-4,9 (13)-dien-3-one (1),3β-hydroxy-β-ionone (2),3α-hydroxymegastigma-4,7-E-dien-9-one (3),5α,6α-epoxy-3α-hydroxy-megastigma-7E-en-9-one (4),loliolide (5),pisiferadinol (6),24-methylenecycloartanol (7),α-amyrin (8),3β-hydroxyurs-11-en-28,13-β-olide (9),lupeol (10),achilleol A (11),6β-methoxyergosta-7,22-E-dien-3β,5α-diol (12),6β-ethoxyergosta-7,22-E-dien-3β,5α-diol (13),stigmast-4-en-3,6-dione (14),stigmast-4,22-E-dien-3,6-dione (15),by modern spectroscopic methods.Compound 1 was isolated from nature resources for the first time,and compounds 2-15 were obtained from H.triflora for the first time.Compounds 6,12,15 showed significant cytotoxic activities against MDA-MB-468,AGS,HCT116,Hela,and MDA-MB-231 cells.

Key words: Hypoestes triflora, chemical constituent, terpenoid, steroid, cytotoxic activity