Abstract: This study was conducted to investigate the protective effect of swertisin on damage PC12 cells induced by H₂O₂,as well as its primary mechanism.The cell viability was measured by MTT assay.The activity of SOD,CAT and GSH-Px,the level of were measured by the colorimetric method.The cell reactive oxygen species (ROS) was detected by the reactive oxygen test kit.The cell mitochondrial membrane potential was detected by the mitochondrial membrane potential detection kit (JC-1).The investigation showed that swertisin can improve cell viability in damage PC12 cells induced by H₂O₂,inhibit the releasing of MDA and LDH,enhance SOD,CAT and GSH-Px activities,inhibit intracellular ROS increase,and stable mitochondrial membrane potential.The results demonstrated that swertisin can suppress the oxidative damage in PC12 cells induced by H₂O₂ in multiple levels,which play a protective role in nerve cells.

Key words: swertisin, PC12 cell, oxidative damage