Abstract: In order to investigate the metabolic characteristics of the effective components of Polygonum orientale flower in human intestinal flora.The effective components of Polygonum orientale flower were added to in vitro culture of human intestinal bacteria and incubated in anaerobic environment.The metabolites were detected by UPLC-Q-TOF/MS.The metabolites in the samples were predicted and analyzed by using Metabolite Tools^TM analysis platform and the chemical formula of metabolites was deduced by using the Smart Formula function in Data Analysis combined with Mass Defect Filter technology.A total of 14 metabolites were detected from the drug-enteroinoculum solution,including N-p-coumaroyl tyramide,hydrogenation of N-trans-p-hydroxyphenethyl ferulamide,hydrogenation hydroxylation,and methylation.Hydroxylated metabolites,dodeoxylation of kaempferol,reduction of C2-C3 double bonds,deoxygenation metabolites,hydroxylation of quercetin,ring-opening cleavage and deoxidation of O-C2 bonds,and C2-C3 double bond reduction of O-C2 bonds Ring-opening pyrolysis metabolites,hydrogenated metabolites of catechins,and decarboxylated,methylated metabolites of gallic acid.The effective components of Polygonum orientale flower are mainly reduced,oxidized,hydrolyzed I phase reaction and methylated phase II reaction in isolated human intestinal flora,which will provide reference for further study of its mechanism in vivo.

Key words: Polygonum orientale flower effective components, metabolism, intestinal flora, UPLC-MS/MS