Abstract: In order to study the nutritive value of fatty acid in giant salamander oil,the oil was extracted by enzymatic hydrolysis.The gas chromatographymass spectrometry (GC-MS) was used to analyze the changes of fatty acid composition in the crude giant salamander oil and its refining (degumming,deacidification and deodorization) and the difference in fatty acid composition from refined grass carp fish oil.The results showed that the optimum hydrolysis conditions were enzymatic hydrolysis temperature of 60 ℃,pH6.5,enzyme dosage of 0.75% and enzymatic hydrolysis time of 90 min.The obtained crude giant salamander oil meets the secondary standard of crude fish oil as detained in the SC/T 35022016.The results showed that 20 kinds of fatty acids were detected,and the compositions of fatty acids in the crude oil during refining process were basically unchanged.The relative contents of unsaturated fatty acids and essential fatty acids in the refined oil were 74.76% and 25.17%,higher than those of the refined grass carp fish oil (61.08% and 18.90%,separately).It can be seen that the current method is more effective for the extraction of l giant salamander oil,and the nutritional value of giant salamander oil is higher than that of grass carp oil.

Key words: Andrias davidianus oil, extraction aqueous enzymatic hydrolysis, refining, fatty acid