Abstract: This paper took Anemone obtusiloba as materials,used UV-vis spectrophotometry,HPLC and LC-MS analysis on the different flower color of pigment,determination of the major pigments and related synthetase that controled the depth of flower color.The results showed:A.obtusiloba contain two kinds of pigments,carotenoids and flavonoids,the total absorbance of both of them varies with the law of color depth,but with the shallow color flavonoids absorbance change is more obvious,by HPLC-MS analysis 7 kinds of flavonoids were determined:luteolin-3-7-O-glucoside,quercetin-3-O-sophoroside,kaempferol-3-O-sophoroside,myricetin-3-O-rhamnose,quercetin-3-O-galactosidase,quercetin-3-O-glucoside,quercetin-3-O-rhamnoside.The derivative of quercetin is the main pigment that causes the difference of color,the flavonoid3′-hydroxylase catalyze its synthesis is the key enzyme.Infer that the mechanism for adapting the color of A.obtusiloba to the environment through flavonoid3′-hydroxylase to control the depth of color.

Key words: A.obtusiloba, anthocyanins, HPLC, LC-MS