Abstract:

NanoSe-LMH-CS-SA drug-loading microsphere was prepared through the complex coacervation method using chitosan and sodium alginate as the wall materials, NanoSe as the core materials and LMH as the composite nutrient. These properties such as SEM morphology, particle size distribution, infrared spectroscopy, thermal stability and element composition were analyzed to describe the microspheres structure. Based on the single factor in vitro release experiments, the preparation technology optimized by Box-Benhnken response surface design was: the ratio of wall material to wall material of 0.82(n_CS/n_SA=0.82), the ratio of core material to wall material of 1.22(n_NanoSe/(n_CS+n_SA)), adding 0.86 g CaCl₂ as crosslinking agent, the complex coacervation pH ranging was 4.28, and the embedding rate of drug-loading microsphere was 84.65%. Simulated digestion experiments showed that the cumulative rate of slow release of optimized drug-loading microsphere were 73.08% (simulated gastric juice) and 57.95% (simulated small intestinal juice)in 5 h, 78.57% (simulated gastric juice) and 65.80% (simulated small intestinal juice) in 7 h.

Key words:

NanoSe,  , LMH,  , chitosan,  , sodium alginate,  , sustained-release,  , response surface design