Abstract:

To study the relationship between the HPLC fingerprint and the antioxidant activity of the hawthorn leaf standard decoction.In this paper,HPLC was used to determine the fingerprint of 16 batches of hawthorn leaf standard decoction from different producing areas,DPPH free radical scavenging method and ABTS free radical scavenging method were used to determine its antioxidant activity in vitro,and the spectrum effect relationship of hawthorn leaf standard decoction from different producing areas was studied by grey relational degree method.The results showed that hawthorn leaf standard decoction had good antioxidant activity,and the 18 common peaks of hawthorn leaf standard decoction had a high correlation with free radical scavenging,with the correlation coefficient higher than 0.72,the correlation of vitexin glucoside was the highest.In this paper,the fingerprint of hawthorn leaf standard decoction and its antioxidant activity spectral effect relationship were successfully established,which can be used to effectively control the quality standard of hawthorn leaf standard decoction.

Key words: hawthorn leaf, standard decoction, grey relational degree method, spectrum-effect relationship