To explore the effect of Lycium ruthenicum Murray anthocyanidin on proliferation and autophagy of HepG2 cells in vitro,the viability of HepG2 cells were detected by CCK-8.Besides,HepG2 cells proliferation and migration were explored by EdU and cell scratch test.Then RT-PCR and Western blot were performed to explore the expression of proliferation-related genes and autophagy-related genes at mRNA and protein level,respectively.We found that L. ruthenicum anthocyanidin could significantly inhibit the proliferation and migration of HepG2 cells.The mRNA expression of proliferation factors (LATS1,LATS2 and MOB1) and autophagy factors (Beclin-1,LC3-Ⅱ and AMPK) induced by  L. ruthenicum anthocyanidin were up-regulated,while the mRNA expression of proliferation factor YAP induced by  L. ruthenicum anthocyanidin were down-regulated.Furthermore, L. ruthenicum anthocyanidin decreased autophagy factor p-mTOR and cyclin factor CDK4 protein expression,while enhanced autophagy factors (p-AMPK and LC3-Ⅱ) protein expression.In conclusion,L.ruthenicum anthocyanidin can inhibit the proliferation and migration,and promote the autophagy of HepG2 cells in vitro.