Psidii Guajavae Folium(PGF) are dry leaves of Psidium guajava Linn.A plant of Myrtle family,mainly distributed in Guangdong,Guangxi,Fujian,Hainan,Sichuan and Yunnan.In this study,the high performance liquid chromatography (HPLC) fingerprint was used to evaluate the quality of guava leaves from different habitats.Similarity evaluation,cluster analysis,principal component analysis and orthogonal partial least squares-discriminant analysis were adopted to analyze fingerprint data.The results showed that 31 batches of PGF have 14 common peaks with similarity ranged from 0.720 to 0.928,among which PGF with a similarity less than 0.8 were mainly produced in Guangxi and Guangdong,while those with a similarity more than 0.9 were mainly produced in Sichuan and Yunnan.According to the cluster analysis and principal component analysis,the samples of PGF could be roughly divided into two types in terms of the origin,namely coastal areas (Guangxi,Guangdong,Fujian,Hainan) and southwest areas (Sichuan,Yunnan),with differences in the quality of PGF from different origins.Orthogonal partial least squares method was used to screen out 7 components with quality differences,including ellagic acid,isoquercetin,guaijaverin,reynoutrin and hyperoside.According to the quantitative analysis of ellagic acid,isoquercetin,guaijaverin,reynoutrin and hyperoside,the total amount of the five components in southwest China outweighed that in coastal areas,which demonstrates a significant difference.In this study,the method combining the HPLC fingerprint of PGF with chemical measurement was simple and accurate,which could provide references for the quality control and evaluation of PGF.