Valeriana jatamansi Jones,a traditional herbal medicine used by many ethnic groups in China,has a wide range of medicinal value.Studying the active ingredients of V. jatamansi and its mechanism of action is of great significance to the comprehensive development and utilization of V. jatamansi.In this study,RAW264.7 cells treated with lipopolysaccharide (LPS) were used as an inflammatory cell model to investigate the anti-inflammatory activities of 14 iridoids isolated from the dried roots and rhizomes of V. jatamansi.The results showed that compounds 1,2,3,5,8 and 9 could significantly inhibit the production of NO,among which compounds 1 and 2 Chad the most significant inhibitory effect,with IC₅₀ values of 0.88 and 0.62 μmol/L,respectively.Further experiments showed that compounds 1 and 2 could inhibit the secretion of TNF-α,IL-1β and IL-6,down-regulate the expression of iNOS,COX-2,NLRP3 and the phosphorylation levels of IκBα,STAT3 and mTOR in LPS-induced RAW264.7 cells,but had no significant effect on the MAPKs protein family.In summary,compounds 1 and 2 may reduce the expression of inflammation-related proteins and the secretion of inflammatory factors by down-regulating NF-κB and mTOR/STAT3 signaling pathways,thereby reducing the LPS-induced inflammatory response of macrophages.