To extend life span of cell membrane material,a novel type of 3-aminopropyltriethoxysilane (APTES) decorated silica gel was employed. A L02 hepatic steatosis cell model was established using oleic acid.Silica gel was decorated with glutaraldehyde with the help of APTES,which react with the amino groups on L02 hepatic steatosis cell membranes to form a covalent bond.For the material characterization was used Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).The material was selected as an adsorptive material to screen for the potential active ingredients of Rheum palmatum L.by HPLC.SEM confirmed that the surface of silica gel was obviously covered with cell membrane.The characteristic absorption peaks of -NH bond were 3 442 and 2 942 cm^-1,indicating that the material was successfully prepared.HPLC analysis showed that 11 chemical components were specifically adsorbed,which were identified as aloe-emodin-8-O-β-D-glucopyranoside,rhein-8-O-β-D-glucopyranoside,emodin-8-O-β-D-glucoside,physcion-8-O-β-D-monoglucoside, aloe-emodin, rhein,emodin,chrysophanol,physcion and two unknown components.The chemically modified L02 liver membrane bioaffinity material can prolong its life span and use for rapid screening of the lipid-lowering active ingredients of Rheum palmatum L.and other traditional Chinese medicine.