The ethanol extracts of Dendrobium officinale from different habitats were analyzed by UPLC-Q-TOF-MS.Data analysis software combined with local database was used to analyse the chemical composition.A total of 32 compounds including (-)-lirioresinol B,fructose phenylalanine,vanillic acid,p-hydroxybenzoic acid and dendrobine were identified,among which 13 compounds were common components.The contents of gigantol and naringin,the two main components of D. officinale,were quantitatively analyzed.The results showed that the contents of the two compounds were different in the ethanol extracts of D. officinale from different habitats.The content of dendrobiol ranged from 0.080 0 to 9.593 5 mg/g,and the content of naringin ranged from 0.032 1 to 0.187 3 mg/g.The results showed that these two compounds could be used as potential quality evaluation indexes of D. officinale. This experiment conducted a comprehensive and systematic analysis of the chemical components in D. officinale from the main production areas in China.The results confirmed the accuracy of previous studies,and further proposed that the two types of compounds are potential new indicators to improve the existing quality evaluation standards of D. officinale.