The UPLC fingerprint of Rosae Chinensis Flos (RCF) and the determination methods of hyperoside and isoquercitrin in RCF were established to evaluate the quality of roses from different habitats and applied to the comparative study of Rose Rugosae Flos(RRF) from the same family.UPLC was used to establish the fingerprint of RCF,and the common peaks were matched,and the attribution of each common peak was analyzed.SPSS 20.0 and SIMCA 14.1 software were used for cluster analysis and principal component analysis of the data.The total contents of hyperoside and isoquercitrin in roses and the fingerprints of RRF were determined by the same method.The UPLC fingerprint of RCF was established to calibrate 30 common peaks,and 10 components were identified by comparison with reference substances;Cluster analysis showed that when the clustering distance was 10,15 batches of RCF could be divided into five categories,and the results of PCA and cluster analysis were basically the same;Content determination showed that the total content of hyperoside and isoquercitrin in 15 batches of RCF were 3.88-8.67 mg/g,indicating that there were some differences in the total content of hyperoside and isoquercitrin in different habitats;The established fingerprint method is also suitable for the quality control of RRF in the same family.The established fingerprint and content determination method of RCF were feasible and could provide reference for the follow-up study of RCF.