Through network pharmacology and in vitro experiments,the active ingredients and mechanism of oil peony for anti-inflammatory were analyzed.ADME screening was carried out on the oil peony components obtained from TCMSP database and 91 active ingredients and 716 targets were obtained.A total of 1 009 targets of skin inflammation were obtained from the disease database.Taking the intersection of the two,152 anti-inflammatory targets of peony oil were obtained and the PPI network of them was constructed.The core targets involved TNF-α,IL-6,PTGS2 and so on.GO and KEGG enrichment analysis of oil peony anti-inflammatory targets mainly involved in biological processes such as the response to inflammation and the response to bacteria derived from molecules.The RAW 264.7 inflammatory cell model induced by lipopolysaccharide was used in the in vitro test,and the effects of different concentrations of oil peony extract on the secretion of TNF-α,IL-6,NO,and COX-2 were detected.The results showed that the concentrations of 1%,0.5%,0.25% and 0.13% could inhibit the expression of them in the inflammatory injury models.