The secondary metabolites of Streptomyces bobili K6,a pathogen of potato scab in Xinjiang,were isolated by silica gel column chromatography and preparative HPLC. The fermentation broth of strain K6 yielded five monoterpene compounds,which were identified as p-methoxybenzoic acid (1),N-acetylhomocysteine thiolactone (2),1-(1H-indol-5-yl)ethanone (3),phenazine-1-carboxamide (4),and 4-deoxy-ε-pyrromycinone (5) through mass spectrometry and spectroscopy analysis. Notably,compound 3 was reported here for the first time as a natural product. Antibacterial assays revealed that compound 4 exhibited potent activity against Staphylococcus epidermidis,with a minimum inhibitory concentration(MIC) of 12.5 μg/mL. Furthermore,compound 5 demonstrated antifungal activity against Candida albicans,with an MIC value of 25 μg/mL. In terms of antitumor effects,compound 5 displayed moderate inhibition on the proliferation of HCT-116 colon cancer cells and MDA-MB-231 breast cancer cells,resulting in inhibition rates of 88.63% and 80.96%,respectively. This study lays the foundation for further development of novel antibacterial agents and anticancer drugs derived from Streptomyces.