To explore the bioactive constituents of Sanghuangporus sanghuang body ethanolic extract (SSBEE) and its anti-non-small cell lung cancer (NSCLC) effect and underlying mechanism of action,this study first used UHPLC-Q-Exactive-Orbitrap-MS technology to detect the main active components of SSBEE.Secondly,Swiss ADME and Swiss Target Prediction databases were used to determine the potential active components and targets of SSBEE,and GeneCards database was used to screen disease genes and perform GO and KEGG enrichment analysis on core targets.A key network diagram was constructed.Meanwhile,molecular docking and molecular dynamics were used to evaluate the binding between the core components of SSBEE and potential targets.Finally,CCK-8 and Western blot assays were used to evaluate the anti-NSCLC activity and possible mechanism of action of SSBEE.A total of 54 compounds were identified from SSBEE,among which 19 active compounds mainly acted on 15 core targets such as PIK3CA and CTNNB1.KEGG enrichment analysis found that it may play an anti-NSCLC role through multiple pathways such as HIF-1 and PI3K-Akt.Molecular docking and molecular dynamics showed that ulocladol and genistein had good affinity for core targets such as PIK3CA protein.Functional verification experiments showed that SSBEE (160 μg/mL) had an inhibition rate of 81.26% on A549,which could inhibit the expression of target proteins PIK3CA and CTNNB1.This study clarified the main active components and potential mechanism of action of the ethanol extract of the fruiting body of S. sanghuang,providing a scientific theoretical basis for the prevention and treatment of NSCLC with S. sanghuang.

"> network pharmacology, Sanghuangporus sanghuang, molecular docking, molecular dynamics, non-small cell lung cancer, UHPLC-Q-Exactive-Orbitrap-MS