Abstract:

This study aims to establish a quantitative control method for multi-index components of Sambuci Williamsii Ramulus, and to evaluate the quality difference of Sambuci Williamsii Ramulus from different producing areas by chemometrics,weighted technique for order preference by similarity to ideal solution and grey relational analysis fusion model for improving the overall quality control level of Sambuci Williamsii Ramulus medicinal materials.The analysis was performed on a Discovery C₁₈ column,with a mobile phase consisting of acetonitrile-0.1% phosphoric acid for gradient elution.The relative correction factors of quercetin and gallic acid,protocatechuic acid,rutoside,isoquercitrin,cyanidin-3-O-glucoside chloride,betulinic acid,morroniside,oleanolic acid,ursolic acid,balanophonin and lariciresinol were calculated,the content of each component was determined by the mean of the relative correction factor,and the difference between the external standard method and the quantitative analysis of multi-components by single marker method was compared.The extract and total ash were detected according to the standard of Pharmacopoeia of People′s Republic of China.principal component analysis and orthogonal partial least squares discriminant analysis were performed on the test results of each evaluation index of 18 batches of Sambuci Williamsii Ramulus to explore the differential markers affecting product quality.A weighted TOPSIS and GRA fusion model was established to comprehensively evaluate the quality of Sambuci Williamsii Ramulus from different producing areas.The linear relationship of 12 components (R²> 0.999 2),instrument precision,repeatability and stability (24 h) were good.The average recover (n=9) was between 96.95% and 100.1% (relative standard deviation< 2.0%).The relative correction factor had good robustness,and there was little difference between the two methods.The PCA results showed that 18 batches of Sambuci Williamsii Ramulus were clustered into 3 categories.The results of OPLS-DA showed that balanophonin,oleanolic acid,betulinic acid,isoquercitrin,gallic acid and cyanidin-3-O-glucoside chloride were the differential markers affecting the quality of Sambuci Williamsii Ramulus.The relative closeness of 18 batches of samples ranged from 0.236 2 to 0.717 2,indicating that there were regional differences in Sambuci Williamsii Ramulus.The established method is convenient,economical,accurate and practical,and can realize the rapid analysis of multi-index components of Sambuci Williamsii Ramulus.Chemometrics combined with weighted TOPSIS and GRA fusion model can be used for the overall quality evaluation of Sambuci Williamsii Ramulus in different producing areas.

Key words: Sambuci Williamsii Ramulus, quantitative analysis of multi-components by single marker, chemometrics, weighted TOPSIS, grey relational analysis, quality evaluation