Abstract:

This study aims to compare the effects of honey processed Hedysari Radix-vinegar-processed Curcumae Rhizoma (HPHR-VPCR) and Hedysari Radix-vinegar-processed Curcumae Rhizoma (HR-VPCR) on apoptosis and autophagy in A549 cells and NCI-H1299 cells, and explore the mechanism of HPHR-VPCR inducing apoptosis and autophagy. Twenty-one SPF grade SD rats were randomly divided into three groups: control (Con) group, HR-VPCR group, and HPHR-VPCR group. The dosage of each drug pair was 4.63 g/kg, and the drug containing serum was taken after continuous administration for seven days; Hoechst33258 staining was used to detect cell apoptosis morphology, and flow cytometry was used to detect cell apoptosis rate; The monodansylcadaverine (MDC) method was used to detect cellular autophagy; Transmission electron microscopy was used to detect autophagosomes and autolysosomes; Western blot was used to detect the expression of ubiquitin-binding protein P62 (P62), beclin1(BECN1), B-cell lymphoma-2 (BCL-2), and BCL-2 associated X protein (BAX) proteins. The results of Hoechst33258 and flow cytometry analysis showed that in the groups treated with HR-VPCR and HPHR-VPCR, chromatin accumulation, nuclear concentration and fragmentation were severe, apoptosis fluorescence was enhanced, and the proportion of apoptosis increased; The MDC method detection results showed that the green fluorescence of the HR-VPCR group and HPHR-VPCR group was enhanced, and autophagosomes were increased; The Western blot results showed that compared with the blank group, the expression of P62 and BCL-2 proteins was significantly reduced in HPHR-VPCR group, while the expression of BECN1 and BAX proteins was significantly increased (P<0.01, P<0.05). In summary, both HR-VPCR and HPHR-VPCR can induce cell apoptosis and autophagy. HPHR-VPCR is superior to HR-VPCR in inducing cell apoptosis and autophagy. The induction of apoptosis and autophagy in lung cancer cells by HPHR-VPCR is related to the decrease of P62 and BCL-2, as well as the increase of BECN1 and BAX expression.

Key words:

Hedysari Radix-vinegar-processed Curcumae Rhizoma, honey-processed Hedysari Radix-vinegar-processed Curcumae Rhizoma; lung cancer cells, apoptosis, autophagy