Abstract: To establish an UPLC method for simultaneous determination of six compounds,namely aucubin,geniposidic acid,chlorogenic acid,geniposide,pinoresinol diglucoside and pinoresinol singleglucoside in Eucommia ulmoides.UPLC analysis was carried out on Waters BET C18 column (2.1×150 mm,1.7 μm),with the mobile phase of 0.1% formic acid acetonitrile solution 0.1% formic acid aqueous solution in gradient elution mode.The flow rate was 0.25 mL/min.The UV detection wavelength was 205 nm.The column temperature was 45 ℃.The six components of E.ulmoides can be separated in the chromatographic conditions.The recoveries of six components were 96.92%-101.01%,with RSD less than 3%.The total content of six components from 39 batch E.ulmoides were 0.4406%-4.2282%.The developed UPLC method was simple,accurate and highly reproducible.It can be used for the simultaneous detection and determination of aucubin,geniposidic acid,chlorogenic acid,geniposide,pinoresinol diglucoside and pinoresinol singleglucoside.

Key words: Eucommia ulmoides, UPLC, aucubin, geniposidic acid, chlorogenic acid, geniposide, pinoresinol diglucoside, pinoresinol singleglucosid